Mouse MOG Gene cDNA Clone / ORF Clone

MOG cDNA Clone Datasheet

MOG cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010814.2
• cDNA Size: 745
• Gene Synonym: B230317G11Rik; Mog
• Source: Mouse

MOG cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus myelin oligodendrocyte glycoprotein DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 745 G/A not causing the amino acid variation, and without Stop codon.

MOG cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MOG cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MOG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTGTT TGTGGAGCTT CTCTTGGCCC AGCTGCTTCC TCTCCCTTCT CCTCCTCCTT
61       CTCCTCCAGT TGTCATGCAG CTATGCAGGA CAATTCAGAG TGATAGGACC AGGGTATCCC
121      ATCCGGGCTT TAGTTGGGGA TGAAGCAGAG CTGCCGTGCC GCATCTCTCC TGGGAAAAAT
181      GCCACGGGCA TGGAGGTGGG TTGGTACCGT TCTCCCTTCT CAAGAGTGGT TCACCTCTAC
241      CGAAATGGCA AGGACCAAGA TGCAGAGCAA GCACCTGAAT ACCGGGGACG CACAGAGCTT
301      CTGAAAGAGA CTATCAGTGA GGGAAAGGTT ACCCTTAGGA TTCAGAACGT GAGATTCTCA
361      GATGAAGGAG GCTACACCTG CTTCTTCAGA GACCACTCTT ACCAAGAAGA GGCAGCAATG
421      GAGTTGAAAG TGGAAGATCC CTTCTATTGG GTCAACCCCG GTGTGCTGAC TCTCATCGCA
481      CTTGTGCCTA CGATCCTCCT GCAGGTCTCT GTAGGCCTTG TATTCCTCTT CCTGCAGCAC
541      AGACTGAGAG GAAAACTTCG TGCAGAAGTA GAGAATCTCC ATCGGACTTT TGATCCTCAC
601      TTCCTGAGGG TGCCCTGCTG GAAGATAACA CTGTTTGTTA TTGTGCCTGT TCTTGGACCC
661      CTGGTTGCCT TGATCATCTG CTACAACTGG CTGCACCGAA GACTGGCAGG ACAGTTTCTT
721      GAAGAGCTAA GAAACCCCTT TTGAA

Quality Control:cDNA sequencing results is available upon request.