Mouse MMP14 Gene cDNA Clone / ORF Clone

MMP14 cDNA Clone Datasheet

MMP14 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008608.3
• cDNA Size: 1749
• Gene Synonym: MT1-MMP; AI325305; MT-MMP-1; Mmp14
• Source: Mouse

MMP14 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus matrix metallopeptidase 14 (membrane-inserted) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 540 G/A,750 C/T,753 T/C,1119 G/A,1218 G/T,1392 G/A,1491 T/C and 1623 C/T not causing the amino acid variation.

MMP14 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MMP14 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MMP14 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTCCCG CCCCTCGACC CTCCCGCAGC CTCCTGCTCC CCCTGCTCAC GCTTGGCACG
61       GCGCTCGCCT CCCTCGGCTG GGCCCAAGGC AGCAACTTCA GCCCCGAAGC CTGGCTGCAG
121      CAGTATGGCT ACCTACCTCC AGGGGACCTG CGTACCCACA CACAACGCTC ACCCCAGTCA
181      CTCTCAGCTG CCATTGCCGC CATGCAAAAG TTCTATGGTT TACAAGTGAC AGGCAAGGCT
241      GATTTGGCAA CCATGATGGC CATGAGGCGC CCTCGCTGTG GTGTTCCGGA TAAGTTTGGG
301      ACTGAGATCA AGGCCAATGT TCGGAGGAAG CGCTATGCCA TTCAGGGCCT CAAGTGGCAG
361      CATAATGAGA TCACTTTCTG CATTCAGAAT TACACCCCTA AGGTGGGCGA GTATGCCACA
421      TTCGAGGCCA TTCGGAAGGC CTTCCGAGTA TGGGAGAGTG CCACGCCACT GCGCTTCCGA
481      GAAGTGCCCT ATGCCTACAT CCGGGAGGGA CATGAGAAGC AGGCTGACAT CATGATCTTA
541      TTTGCTGAGG GTTTCCACGG CGACAGTACA CCCTTTGATG GTGAAGGAGG GTTCCTGGCT
601      CATGCCTACT TCCCAGGCCC CAATATTGGA GGGGATACCC ACTTTGATTC TGCCGAGCCC
661      TGGACTGTCC AAAATGAGGA TCTAAATGGG AATGACATCT TCTTGGTGGC TGTGCATGAG
721      TTGGGGCATG CCCTAGGCCT GGAACATTCT AACGATCCCT CCGCCATCAT GGCCCCCTTT
781      TACCAGTGGA TGGACACAGA GAACTTCGTG TTGCCTGATG ACGATCGCCG TGGCATCCAG
841      CAACTTTATG GAAGCAAGTC AGGGTCACCC ACAAAGATGC CCCCTCAACC CAGAACTACC
901      TCTCGGCCCT CTGTCCCAGA TAAGCCCAAA AACCCCGCCT ATGGGCCCAA CATCTGTGAC
961      GGGAACTTTG ACACCGTGGC CATGCTCCGA GGAGAGATGT TTGTCTTCAA GGAGCGATGG
1021     TTCTGGCGGG TGAGGAATAA CCAAGTGATG GATGGATACC CAATGCCCAT TGGCCAATTC
1081     TGGAGGGGCC TGCCTGCATC CATCAATACT GCCTACGAAA GGAAGGATGG CAAATTTGTC
1141     TTCTTCAAAG GAGATAAGCA CTGGGTGTTT GACGAAGCCT CCCTGGAACC CGGGTACCCC
1201     AAGCACATTA AGGAGCTTGG CCGAGGGCTG CCCACGGACA AGATCGATGC AGCTCTCTTC
1261     TGGATGCCCA ATGGGAAGAC CTACTTCTTC CGGGGCAATA AGTACTACCG GTTCAATGAA
1321     GAATTCAGGG CAGTGGACAG CGAGTACCCT AAAAACATCA AAGTCTGGGA AGGAATCCCT
1381     GAATCTCCCA GAGGGTCATT CATGGGCAGT GATGAAGTCT TCACATACTT CTACAAGGGA
1441     AACAAATACT GGAAGTTCAA CAACCAGAAG CTGAAGGTAG AGCCAGGGTA CCCCAAGTCA
1501     GCTCTGCGGG ACTGGATGGG CTGCCCTTCG GGGGGCCGGC CCGATGAGGG GACTGAGGAG
1561     GAGACGGAGG TGATCATCAT TGAGGTGGAT GAGGAGGGCA GTGGAGCTGT GAGTGCGGCC
1621     GCTGTGGTCC TGCCGGTACT ACTGCTGCTC CTGGTACTGG CAGTGGGCCT CGCTGTCTTC
1681     TTCTTCAGAC GCCATGGGAC GCCCAAGCGA CTGCTTTACT GCCAGCGTTC GCTGCTGGAC
1741     AAGGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.