Mouse MMP-9 Gene cDNA Clone / ORF Clone

MMP9 cDNA Clone Datasheet

MMP9 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013599.2
• cDNA Size: 2193
• Gene Synonym: Clg4b; MMP-9; B/MMP9; AW743869; pro-MMP-9; Mmp9
• Source: Mouse

MMP9 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus matrix metallopeptidase 9 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for five point mutations: 1540 C/G resulting in the amino acid Pro substitution by Ala, 1916 T/C resulting in the amino acid Leu substitution by Pro, 2132 A/C resulting in the amino acid His substitution by Pro, 834 G/A and 1962 C/T not causing the amino acid variation.

MMP9 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MMP9 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MMP9 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTCCCT GGCAGCCCCT GCTCCTGGCT CTCCTGGCTT TCGGCTGCAG CTCTGCTGCC
61       CCTTACCAGC GCCAGCCGAC TTTTGTGGTC TTCCCCAAAG ACCTGAAAAC CTCCAACCTC
121      ACGGACACCC AGCTGGCAGA GGCATACTTG TACCGCTATG GTTACACCCG GGCCGCCCAG
181      ATGATGGGAG AGAAGCAGTC TCTACGGCCG GCTTTGCTGA TGCTTCAGAA GCAGCTCTCC
241      CTGCCCCAGA CTGGTGAGCT GGACAGCCAG ACACTAAAGG CCATTCGAAC ACCACGCTGT
301      GGTGTCCCAG ACGTGGGTCG ATTCCAAACC TTCAAAGGCC TCAAGTGGGA CCATCATAAC
361      ATCACATACT GGATCCAAAA CTACTCTGAA GACTTGCCGC GAGACATGAT CGATGACGCC
421      TTCGCGCGCG CCTTCGCGGT GTGGGGCGAG GTGGCACCCC TCACCTTCAC CCGCGTGTAC
481      GGACCCGAAG CGGACATTGT CATCCAGTTT GGTGTCGCGG AGCACGGAGA CGGGTATCCC
541      TTCGACGGCA AGGACGGCCT TCTGGCACAC GCCTTTCCCC CTGGCGCCGG CGTTCAGGGA
601      GATGCCCATT TCGACGACGA CGAGTTGTGG TCGCTGGGCA AAGGCGTCGT GATCCCCACT
661      TACTATGGAA ACTCAAATGG TGCCCCATGT CACTTTCCCT TCACCTTCGA GGGACGCTCC
721      TATTCGGCCT GCACCACAGA CGGCCGCAAC GACGGCACGC CTTGGTGTAG CACAACAGCT
781      GACTACGATA AGGACGGCAA ATTTGGTTTC TGCCCTAGTG AGAGACTCTA CACAGAGCAC
841      GGCAACGGAG AAGGCAAACC CTGTGTGTTC CCGTTCATCT TTGAGGGCCG CTCCTACTCT
901      GCCTGCACCA CTAAAGGCCG CTCGGATGGT TACCGCTGGT GCGCCACCAC AGCCAACTAT
961      GACCAGGATA AACTGTATGG CTTCTGCCCT ACCCGAGTGG ACGCGACCGT AGTTGGGGGC
1021     AACTCGGCAG GAGAGCTGTG CGTCTTCCCC TTCGTCTTCC TGGGCAAGCA GTACTCTTCC
1081     TGTACCAGCG ACGGCCGCAG GGATGGGCGC CTCTGGTGTG CGACCACATC GAACTTCGAC
1141     ACTGACAAGA AGTGGGGTTT CTGTCCAGAC CAAGGGTACA GCCTGTTCCT GGTGGCAGCG
1201     CACGAGTTCG GCCATGCACT GGGCTTAGAT CATTCCAGCG TGCCGGAAGC GCTCATGTAC
1261     CCGCTGTATA GCTACCTCGA GGGCTTCCCT CTGAATAAAG ACGACATAGA CGGCATCCAG
1321     TATCTGTATG GTCGTGGCTC TAAGCCTGAC CCAAGGCCTC CAGCCACCAC CACAACTGAA
1381     CCACAGCCGA CAGCACCTCC CACTATGTGT CCCACTATAC CTCCCACGGC CTATCCCACA
1441     GTGGGCCCCA CGGTTGGCCC TACAGGCGCC CCCTCACCTG GCCCCACAAG CAGCCCGTCA
1501     CCTGGCCCTA CAGGCGCCCC CTCACCTGGC CCTACAGCGG CCCCTACTGC GGGCTCTTCT
1561     GAGGCCTCTA CAGAGTCTTT GAGTCCGGCA GACAATCCTT GCAATGTGGA TGTTTTTGAT
1621     GCTATTGCTG AGATCCAGGG CGCTCTGCAT TTCTTCAAGG ACGGTTGGTA CTGGAAGTTC
1681     CTGAATCATA GAGGAAGCCC ATTACAGGGC CCCTTCCTTA CTGCCCGCAC GTGGCCAGCC
1741     CTGCCTGCAA CGCTGGACTC CGCCTTTGAG GATCCGCAGA CCAAGAGGGT TTTCTTCTTC
1801     TCTGGACGTC AAATGTGGGT GTACACAGGC AAGACCGTGC TGGGCCCCAG GAGTCTGGAT
1861     AAGTTGGGTC TAGGCCCAGA GGTAACCCAC GTCAGCGGGC TTCTCCCGCG TCGTCCCGGG
1921     AAGGCTCTGC TGTTCAGCAA GGGGCGTGTC TGGAGATTCG ATTTGAAGTC TCAGAAGGTG
1981     GATCCCCAGA GCGTCATTCG CGTGGATAAG GAGTTCTCTG GTGTGCCCTG GAACTCACAC
2041     GACATCTTCC AGTACCAAGA CAAAGCCTAT TTCTGCCATG GCAAATTCTT CTGGCGTGTG
2101     AGTTTCCAAA ATGAGGTGAA CAAGGTGGAC CCTGAGGTGA ACCAGGTGGA CGACGTGGGC
2161     TACGTGACCT ACGACCTCCT GCAGTGCCCT TGA

 

Quality Control:cDNA sequencing results is available upon request.