+86-400-890-9989
Product Catalog
> ELISA > ELISA Pair Set > MMP-9 / CLG4B / MMP9 ELISA Pair Set, Human ELISA Pair Set MMP-9 / CLG4B / MMP9 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10327 |
|
YES | 
|
    |
|
Human MMP-9 / CLG4B / MMP9 ELISA Pair Set
 |
Order or Inquire for Human MMP-9 / CLG4B / MMP9 ELISA Pair Set |
|
|
High affinity Human MMP-9 / CLG4B / MMP9 ELISA Pair Set |
|
|
Detection limit - 31.25 pg/ml |
|
|
Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
|
|
|
|
|
No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
|
Substrate solution was not added |
Add substrate solution and continue |
|
|
Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
|
|
Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
|
Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
|
|
Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
|
|
Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
|
|
Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
|
Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
|
|
High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
|
Increase cycles of washes and soaking time between washes |
||
|
TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
|
|
Materials were contaminated |
Use clean plates, tubes and pipettes tips |
|
|
Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
|
The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human MMP-9 / CLG4B / MMP9 ELISA Pair Set Related Products & Topics
Related Areas:
Enzyme>>Protease & Regulator>>Metalloprotease & Regulator>>Matrix Metalloproteinase>>MMP-9/MMP9
Cancer>>Angiogenesis>>Matrix Metalloproteinase>>MMP-9/MMP9
Cancer>>Cancer Biomarkers>>MMP-9/MMP9
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| MMP-9/MMP9 | Human | MMP-9/MMP9 Protein, Recombinant |
10327-H08H |
| MMP-9/MMP9 | Human | MMP-9/MMP9 Protein, Recombinant |
10327-HNAH |
| MMP-9/MMP9 | Mouse | MMP-9/MMP9 Protein, Recombinant | 50560-MNAH |
| MMP-9/MMP9 | Rat | MMP-9/MMP9 Protein, Recombinant |
80049-R08H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human MMP-9/MMP9 | ELISA | Mouse Monoclonal Antibody | 10327-MM01 |
| Human MMP-9/MMP9 | WB | Mouse Monoclonal Antibody | 10327-MM02 |
| Human MMP-9/MMP9 | ELISA | Rabbit Monoclonal Antibody | 10327-RM04 |
| Human MMP-9/MMP9 | WB, ELISA | Rabbit Monoclonal Antibody | 10327-RM05 |
| Human MMP-9/MMP9 | WB, ELISA | MMP-9/MMP9 Antibody, Rabbit PMb (Biotin conjugated) | 10327-R043B |
| Human MMP-9/MMP9 | WB, ELISA | Rabbit Polyclonal Antibody | 10327-RP03 |
| Human MMP-9/MMP9 | WB, ELISA | Rabbit Polyclonal Antibody (Antigen Affinity Purified) | 10327-RP06 |
Background
Matrix metalloproteinase-9, also known as 92 kDa type I V collagenase, MMP-9 and CLG4B is a secreted protein which belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP-9 contains three fibronectin type-II domains and four hemopexin-like domains. It is secreted from neutrophils, macrophages, and a number of transformed cells. It is the most complex family member in terms of domain structure and regulation of its activity. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.
MMP-9 may play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. MMP-9 could play a role in bone osteoclastic resorption. It cleaves type I V and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP-9 also degrades fibronectin but not laminin or Pz-peptide. MMP-9 and MMP-13 catalyze the degradation of extracellular matrix (ECM) components in the growth plate and at the same time cleave and release biologically active molecules stored in the ECM, such as VEGFA. In mice, ablation of MMP-9, MMP-13, or both MMP-9 and MMP-13 causes severe distortion of the metaphyseal growth plate. MMP-9 has also been implicated in numerous somatic illnesses, including cardiovascular disorders and cancer. MMP-9 has been shown to be increasingly important in several aspects of central nervous system activity. Defects in MMP-9 may be a cause of susceptibility to intervertebral disc disease (IDD), also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
