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Human MMP-8 Gene cDNA Clone / ORF Clone
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10254-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_002424.2
cDNA Size: 1404
Synonym: MMP8, HNC, CLG1, PMNL-CL
Source: Human. cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 8 (neutrophil collagenase) DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 95C/T and 259A/G which cause the 32Thr and 87Lys to be replaced by Ile and Glu respectively. 10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
Quality Control: cDNA sequencing results is available upon request.
Gene Bank Ref.ID: NM_002424.2
cDNA Size: 1404
Synonym: MMP8, HNC, CLG1, PMNL-CL
Source: Human. cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 8 (neutrophil collagenase) DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 95C/T and 259A/G which cause the 32Thr and 87Lys to be replaced by Ile and Glu respectively. 10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95℃ for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGTTCTCCC TGAAGACGCT TCCATTTCTG CTCTTACTCC ATGTGCAGAT TTCCAAGGCC 61 TTTCCTGTAT CTTCTAAAGA GAAAAATACA AAAATTGTTC AGGACTACCT GGAAAAGTTC 121 TACCAATTAC CAAGCAACCA GTATCAGTCT ACAAGGAAGA ATGGCACTAA TGTGATCGTT 181 GAAAAGCTTA AAGAAATGCA GCGATTTTTT GGGTTGAATG TGACGGGGAA GCCAAATGAG 241 GAAACTCTGG ACATGATGGA AAAGCCTCGC TGTGGAGTGC CTGACAGTGG TGGTTTTATG 301 TTAACCCCAG GAAACCCCAA GTGGGAACGC ACTAACTTGA CCTACAGGAT TCGAAACTAT 361 ACCCCACAGC TGTCAGAGGC TGAGGTAGAA AGAGCTATCA AGGATGCCTT TGAACTCTGG 421 AGTGTTGCAT CACCTCTCAT CTTCACCAGG ATCTCACAGG GAGAGGCAGA TATCAACATT 481 GCTTTTTACC AAAGAGATCA CGGTGACAAT TCTCCATTTG ATGGACCCAA TGGAATCCTT 541 GCTCATGCCT TTCAGCCAGG CCAAGGTATT GGAGGAGATG CTCATTTTGA TGCCGAAGAA 601 ACATGGACCA ACACCTCCGC AAATTACAAC TTGTTTCTTG TTGCTGCTCA TGAATTTGGC 661 CATTCTTTGG GGCTCGCTCA CTCCTCTGAC CCTGGTGCCT TGATGTATCC CAACTATGCT 721 TTCAGGGAAA CCAGCAACTA CTCACTCCCT CAAGATGACA TCGATGGCAT TCAGGCCATC 781 TATGGACTTT CAAGCAACCC TATCCAACCT ACTGGACCAA GCACACCCAA ACCCTGTGAC 841 CCCAGTTTGA CATTTGATGC TATCACCACA CTCCGTGGAG AAATACTTTT CTTTAAAGAC 901 AGGTACTTCT GGAGAAGGCA TCCTCAGCTA CAAAGAGTCG AAATGAATTT TATTTCTCTA 961 TTCTGGCCAT CCCTTCCAAC TGGTATACAG GCTGCTTATG AAGATTTTGA CAGAGACCTC 1021 ATTTTCCTAT TTAAAGGCAA CCAATACTGG GCTCTGAGTG GCTATGATAT TCTGCAAGGT 1081 TATCCCAAGG ATATATCAAA CTATGGCTTC CCCAGCAGCG TCCAAGCAAT TGACGCAGCT 1141 GTTTTCTACA GAAGTAAAAC ATACTTCTTT GTAAATGACC AATTCTGGAG ATATGATAAC 1201 CAAAGACAAT TCATGGAGCC AGGTTATCCC AAAAGCATAT CAGGTGCCTT TCCAGGAATA 1261 GAGAGTAAAG TTGATGCAGT TTTCCAGCAA GAACATTTCT TCCATGTCTT CAGTGGACCA 1321 AGATATTACG CATTTGATCT TATTGCTCAG AGAGTTACCA GAGTTGCAAG AGGCAATAAA 1381 TGGCTTAACT GTAGATATGG CTGA
Quality Control: cDNA sequencing results is available upon request.
