MMP-8 / CLG1 Protein (Native)

Matrix Metalloproteinase-8 ( MMP-8 / CLG1 ) Protein

MMP8 / CLG1 Protein Product Information

• Synonym: MMP8 , CLG1, HNC, MMP-8, PMNL-CL
• Protein Construction: A DNA sequence encoding the pro form of human MMP8 ( NP_002415.1 ) ( Phe 21 - Gly 467 ) was expressed and purified, with an initial Met at the N-terminus
• Source: Human
• Expression Host: Human Cells

MMP8 / CLG1 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 ( R&D Systems, Catalog # ES001 )
  The specific activity is > 250 pmoles / min / µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MMP8 consisting of 448 amino acids and has a calculated molecular mass of 52 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MMP-8 protein

MMP8 / CLG1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MMP8 / CLG1 Protein Description

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M10A family. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumour may have a protective effect against lymph node metastasis. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.

References

1. Belotti, D. et al., 2003, Cancer. Res. 63: 5224-9.
2. Mira, E. et al., 2004, J. Cell. Sci. 117:1847-57.
3. Ram, M. et al., 2006, J. Clin. Immunol. 26: 299-307.
4. Gutiérrez-Fernández, A. et al., 2007, FASEB J. 21 (10): 2580-91.
5. Decock, J. et al., 2008, BMC Cancer. 8 :77.
6. Laxton,R.C. et al., 2009, Circ Res.105 (9): 921-9.