MMP-8 / CLG1 Antibody (Antigen Affinity Purified)

Matrix Metalloproteinase-8 Antibody ( Antigen Affinity Purified )

MMP-8 / CLG1 Antibody Product Information

• Immunogen: Recombinant human MMP8 protein ( Catalog#10254-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human MMP8 ( rh MMP8; Catalog#10254-H08H; NP_002415.1 ; Met 1 - Gly 467 ). MMP8 specific IgG was purified by human MMP8 affinity chromatography.

MMP-8 / CLG1 Antibody Usage Guide

• Specificity: Human MMP-8 / CLG1
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human MMP-8 in WB. Using a DAB detection system, the detection limit for human MMP-8 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human MMP-8. The detection limit for human MMP-8 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

MMP-8 / CLG1 Antibody Background

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M10A family. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.

References

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