CCL15 / MIP-5 / NCC3 (aa 46-113) Protein (His Tag) (Cytokine)

Chemokine (C-C motif) ligand 15 Protein Datasheet

CCL15 / MIP-5 / NCC3 Protein Product Information

• Synonym: HCC-2, HMRP-2B, LKN-1, LKN1, MIP-1D, MIP-5, MRP-2B, NCC-3, NCC3, SCYA15, SCYL3, SY15
• Protein Construction: A DNA sequence encoding the amino acids (Ser 46 –Ile 113) of human CCL15 (Q16663-1) was expressed, with a polyhistide tag at the C-terminus.
• Source: Human
• Expression Host: E.coli

CCL15 / MIP-5 / NCC3 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CCL15 (aa 46-113) consists of 79 amino acids and has a calculated molecular mass of 8.9 kDa. It migrates as an approximately 10 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CCL15 / MIP-5 / NCC3 protein

CCL15 / MIP-5 / NCC3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CCL15 / MIP-5 / NCC3 Protein Description

C-C motif chemokine 15, also known as Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, MIP-5, Small-inducible cytokine A15, NCC-3, MIP5 and CCL15, is a secreted protein which belongs to the intercrine beta (chemokine CC) family. CCL15 / MIP5 is most abundant in heart, skeletal muscle and adrenal gland. Lower levels of CCL15 / MIP5 are in placenta, liver, pancreas and bone marrow. CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92) are found in high levels in synovial fluids from rheumatoid patients. CCL15 / MIP5 is a human CC chemokine that activates the receptors, CCR1 and CCR3. Unlike other chemokines, it contains an unusually long N-terminal domain of 31 amino acids preceding the first cysteine residue and a third disulfide bond. CCL15 / MIP5 is a chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. CCL15 / MIP5 functions as an essential factor in the migration of monocytes, lymphocytes, and neutrophils. CCL15 / MIP5 acts mainly via CC chemokine receptor CCR1. CCL15 / MIP5 also binds to CCR3, CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92).

References

1. Youn BS. et al., 1998, Blood. 91: 3118-26.
2. Escher SE. et al., 2004, J Pept Res. 63 (1): 36-47.
3. Berahovich RD. et al., 2005, J Immunol. 174: 7341-51.
4. Lee JS. et al., 2010, Mol Biol Rep. 37 (5): 2149-56.