Human MMP16 Gene cDNA Clone / ORF Clone

MMP16 cDNA Clone Datasheet

MMP16 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005941.4
• cDNA Size: 1824
• Gene Synonym: MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP; DKFZp761D112; MMP16
• Source: Human

MMP16 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 16 (membrane-inserted) DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for ten point mutations: 70 C/T resulting in the amino acid Ala substitution by Val, 509 T/C resulting in the amino acid Val substitution by Ala, 584 A/G resulting in the amino acid Asp substitution by Gly, 917 T/C resulting in the amino acid Val substitution by Ala , 1163 T/A resulting in the amino acid Leu substitution by stop, 1228 A/G resulting in the amino acid Lys substitution by Glu, 1337 A/G resulting in the amino acid Glu substitution by Gly , 960 T/C ,1545 C/T and 1551 A/G not causing the amino acid variation.

MMP16 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MMP16 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

MMP16 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCTTAC TCACATTCAG CACTGGAAGA CGGTTGGATT TCGTGCATCA TTCGGGGGTG
61       TTTTTCTTGT AAACCTTGCT TTGGATTTTA TGTGCTACAG TCTGCGGAAC GGAGCAGTAT
121      TTCAATGTGG AGGTTTGGTT ACAAAAGTAC GGCTACCTTC CACCGACTGA CCCCAGAATG
181      TCAGTGCTGC GCTCTGCAGA GACCATGCAG TCTGCCCTAG CTGCCATGCA GCAGTTCTAT
241      GGCATTAACA TGACAGGAAA AGTGGACAGA AACACAATTG ACTGGATGAA GAAGCCCCGA
301      TGCGGTGTAC CTGACCAGAC AAGAGGTAGC TCCAAATTTC ATATTCGTCG AAAGCGATAT
361      GCATTGACAG GACAGAAATG GCAGCACAAG CACATCACTT ACAGTATAAA GAACGTAACT
421      CCAAAAGTAG GAGACCCTGA GACTCGTAAA GCTATTCGCC GTGCCTTTGA TGTGTGGCAG
481      AATGTAACTC CTCTGACATT TGAAGAAGCT CCCTACAGTG AATTAGAAAA TGGCAAACGT
541      GATGTGGATA TAACCATTAT TTTTGCATCT GGTTTCCATG GGGGCAGCTC TCCCTTTGAT
601      GGAGAGGGAG GATTTTTGGC ACATGCCTAC TTCCCTGGAC CAGGAATTGG AGGAGATACC
661      CATTTTGACT CAGATGAGCC ATGGACACTA GGAAATCCTA ATCATGATGG AAATGACTTA
721      TTTCTTGTAG CAGTCCATGA ACTGGGACAT GCTCTGGGAT TGGAGCATTC CAATGACCCC
781      ACTGCCATCA TGGCTCCATT TTACCAGTAC ATGGAAACAG ACAACTTCAA ACTACCTAAT
841      GATGATTTAC AGGGCATCCA GAAGATATAT GGTCCACCTG ACAAGATTCC TCCACCTACA
901      AGACCTCTAC CGACAGCGCC CCCACACCGC TCTATTCCTC CGGCTGACCC AAGGAAAAAC
961      GACAGGCCAA AACCTCCTCG GCCTCCAACC GGCAGACCCT CCTATCCCGG AGCCAAACCC
1021     AACATCTGTG ATGGGAACTT TAACACTCTA GCTATTCTTC GTCGTGAGAT GTTTGTTTTC
1081     AAGGACCAGT GGTTTTGGCG AGTGAGAAAC AACAGGGTGA TGGATGGATA CCCAATGCAA
1141     ATTACTTACT TCTGGCGGGG CTAGCCTCCT AGTATCGATG CAGTTTATGA AAATAGCGAC
1201     GGGAATTTTG TGTTCTTTAA AGGTAACGAA TATTGGGTGT TCAAGGATAC AACTCTTCAA
1261     CCTGGTTACC CTCATGACTT GATAACCCTT GGAAGTGGAA TTCCCCCTCA TGGTATTGAT
1321     TCAGCCATTT GGTGGGGGGA CGTCGGGAAA ACCTATTTCT TCAAGGGAGA CAGATATTGG
1381     AGATATAGTG AAGAAATGAA AACAATGGAC CCTGGCTATC CCAAGCCAAT CACAGTCTGG
1441     AAAGGGATCC CTGAATCTCC TCAGGGAGCA TTTGTACACA AAGAAAATGG CTTTACGTAT
1501     TTCTACAAAG GAAAGGAGTA TTGGAAATTC AACAACCAGA TACTTAAGGT GGAACCTGGA
1561     TATCCAAGAT CCATCCTCAA GGATTTTATG GGCTGTGATG GACCAACAGA CAGAGTTAAA
1621     GAAGGACACA GCCCACCAGA TGATGTAGAC ATTGTCATCA AACTGGACAA CACAGCCAGC
1681     ACTGTGAAAG CCATAGCTAT TGTCATTCCC TGCATCTTGG CCTTATGCCT CCTTGTATTG
1741     GTTTACACTG TGTTCCAGTT CAAGAGGAAA GGAACACCCC GCCACATACT GTACTGTAAA
1801     CGCTCTATGC AAGAGTGGGT GTGA

 

Quality Control:cDNA sequencing results is available upon request.