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Human MMP-12 Gene cDNA Clone / ORF Clone
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10266-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_002426.4
cDNA Size: 1413
Synonym: MMP12, HME, MME, MGC138506
Source: Human.
cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 12 (macrophage elastase) DNA.
Vector: Vector pMD18-T Simple
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 519 T/C not causing the amino acid variation.
10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95℃ for 15-30 min.
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGAAGTTTC TTCTAATACT GCTCCTGCAG GCCACTGCTT CTGGAGCTCT TCCCCTGAAC 61 AGCTCTACAA GCCTGGAAAA AAATAATGTG CTATTTGGTG AAAGATACTT AGAAAAATTT 121 TATGGCCTTG AGATAAACAA ACTTCCAGTG ACAAAAATGA AATATAGTGG AAACTTAATG 181 AAGGAAAAAA TCCAAGAAAT GCAGCACTTC TTGGGTCTGA AAGTGACCGG GCAACTGGAC 241 ACATCTACCC TGGAGATGAT GCACGCACCT CGATGTGGAG TCCCCGATGT CCATCATTTC 301 AGGGAAATGC CAGGGGGGCC CGTATGGAGG AAACATTATA TCACCTACAG AATCAATAAT 361 TACACACCTG ACATGAACCG TGAGGATGTT GACTACGCAA TCCGGAAAGC TTTCCAAGTA 421 TGGAGTAATG TTACCCCCTT GAAATTCAGC AAGATTAACA CAGGCATGGC TGACATTTTG 481 GTGGTTTTTG CCCGTGGAGC TCATGGAGAC TTCCATGCCT TTGATGGCAA AGGTGGAATC 541 CTAGCCCATG CTTTTGGACC TGGATCTGGC ATTGGAGGGG ATGCACATTT CGATGAGGAC 601 GAATTCTGGA CTACACATTC AGGAGGCACA AACTTGTTCC TCACTGCTGT TCACGAGATT 661 GGCCATTCCT TAGGTCTTGG CCATTCTAGT GATCCAAAGG CCGTAATGTT CCCCACCTAC 721 AAATATGTTG ACATCAACAC ATTTCGCCTC TCTGCTGATG ACATACGTGG CATTCAGTCC 781 CTGTATGGAG ACCCAAAAGA GAACCAACGC TTGCCAAATC CTGACAATTC AGAACCAGCT 841 CTCTGTGACC CCAATTTGAG TTTTGATGCT GTCACTACCG TGGGAAATAA GATCTTTTTC 901 TTCAAAGACA GGTTCTTCTG GCTGAAGGTT TCTGAGAGAC CAAAGACCAG TGTTAATTTA 961 ATTTCTTCCT TATGGCCAAC CTTGCCATCT GGCATTGAAG CTGCTTATGA AATTGAAGCC 1021 AGAAATCAAG TTTTTCTTTT TAAAGATGAC AAATACTGGT TAATTAGCAA TTTAAGACCA 1081 GAGCCAAATT ATCCCAAGAG CATACATTCT TTTGGTTTTC CTAACTTTGT GAAAAAAATT 1141 GATGCAGCTG TTTTTAACCC ACGTTTTTAT AGGACCTACT TCTTTGTAGA TAACCAGTAT 1201 TGGAGGTATG ATGAAAGGAG ACAGATGATG GACCCTGGTT ATCCCAAACT GATTACCAAG 1261 AACTTCCAAG GAATCGGGCC TAAAATTGAT GCAGTCTTCT ACTCTAAAAA CAAATACTAC 1321 TATTTCTTCC AAGGATCTAA CCAATTTGAA TATGACTTCC TACTCCAACG TATCACCAAA 1381 ACACTGAAAA GCAATAGCTG GTTTGGTTGT TAG
Quality Control: cDNA sequencing results is available upon request.
