MMP-12 / MME / HME Protein (His Tag)

Matrix Metalloproteinase-12 Protein

MMP-12 Protein Product Information

• Synonym: HME, MGC138506, MME
• Protein Construction: A DNA sequence encoding the human MMP12 precursor ( NP_002417.2 ) ( Met 1 - Cys 470 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

MMP-12 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 17
• Molecular Mass: The recombinant human MMP12 (pro form) comprises 465 amino acids and has a predicted molecular mass of 53.8 kDa. Probably as a result of autocatalysis, rhMMP12 migrates as an 28 kDa bnd in SDS-PAGE under reducing conditions, corresponding to the active catalytic domain. The 54 kDa pro form is also seen in the SDS-PAGE.
• Formulation: Lyophilized from sterile 25 mM MES, 0.15 M NaCl,10 mM CaCl₂, 0.05% Brij 35, pH 5.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MMP-12 protein

MMP-12 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MMP-12 Protein Description

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Macrophage metalloelastase, also known as Matrix metalloproteinase-12, Macrophage elastase, MMP12, and MMP-12, is a secreted protein which belongs to the peptidase M10A family. MMP12 is a macrophage-secreted elastase that is highly induced in the liver and lung in response to S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme responsible for cleavage of plasminogen to angiotensin, which has an angiostatic effect. It may be involved in tissue injury and remodeling and has significant elastolytic activity. It may be related to prognosis in breast cancer patients. MMP12 promotes fibrosis by limiting the expression of specific ECM-degrading MMPs. Like MMP12, MMP13 expression is highly dependent on IL-13 and type I I-IL-4 receptor signaling. MMP12 is a potent proinflammatory and oncogenic molecule. MMP12 up-regulation plays a critical role in emphysema to lung cancer transition that is facilitated by inflammation.

References

1. Gutiérrez-Fernández, A. et al., 2007, FASEB J. 21 (10): 2580-91.
2. Decock, J. et al., 2008, BMC Cancer. 8 :77.
3. Laxton, RC. et al., 2009, Circ Res. 105 (9): 921-9.
4. Qu, P. et al.,2009, Cancer Res. 69 (18): 7252-61.
5. Madala,SK. et al., 2010, J Immunol. 184 (7): 3955-63.