Human MMP-10 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10249-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002425.2

cDNA Size:    1431

Synonym:   MMP10, SL-2, STMY2

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens matrix metallopeptidase 10 (stromelysin 2) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGATGCATC TTGCATTCCT TGTGCTGTTG TGTCTGCCAG TCTGCTCTGC CTATCCTCTG
61       AGTGGGGCAG CAAAAGAGGA GGACTCCAAC AAGGATCTTG CCCAGCAATA CCTAGAAAAG
121      TACTACAACC TCGAAAAGGA TGTGAAACAG TTTAGAAGAA AGGACAGTAA TCTCATTGTT
181      AAAAAAATCC AAGGAATGCA GAAGTTCCTT GGGTTGGAGG TGACAGGGAA GCTAGACACT
241      GACACTCTGG AGGTGATGCG CAAGCCCAGG TGTGGAGTTC CTGACGTTGG TCACTTCAGC
301      TCCTTTCCTG GCATGCCGAA GTGGAGGAAA ACCCACCTTA CATACAGGAT TGTGAATTAT
361      ACACCAGATT TGCCAAGAGA TGCTGTTGAT TCTGCCATTG AGAAAGCTCT GAAAGTCTGG
421      GAAGAGGTGA CTCCACTCAC ATTCTCCAGG CTGTATGAAG GAGAGGCTGA TATAATGATC
481      TCTTTTGCAG TTAAAGAACA TGGAGACTTT TACTCTTTTG ATGGCCCAGG ACACAGTTTG
541      GCTCATGCCT ACCCACCTGG ACCTGGGCTT TATGGAGATA TTCACTTTGA TGATGATGAA
601      AAATGGACAG AAGATGCATC AGGCACCAAT TTATTCCTCG TTGCTGCTCA TGAACTTGGC
661      CACTCCCTGG GGCTCTTTCA CTCAGCCAAC ACTGAAGCTT TGATGTACCC ACTCTACAAC
721      TCATTCACAG AGCTCGCCCA GTTCCGCCTT TCGCAAGATG ATGTGAATGG CATTCAGTCT
781      CTCTACGGAC CTCCCCCTGC CTCTACTGAG GAACCCCTGG TGCCCACAAA ATCTGTTCCT
841      TCGGGATCTG AGATGCCAGC CAAGTGTGAT CCTGCTTTGT CCTTCGATGC CATCAGCACT
901      CTGAGGGGAG AATATCTGTT CTTTAAAGAC AGATATTTTT GGCGAAGATC CCACTGGAAC
961      CCTGAACCTG AATTTCATTT GATTTCTGCA TTTTGGCCCT CTCTTCCATC ATATTTGGAT
1021     GCTGCATATG AAGTTAACAG CAGGGACACC GTTTTTATTT TTAAAGGAAA TGAGTTCTGG
1081     GCCATCAGAG GAAATGAGGT ACAAGCAGGT TATCCAAGAG GCATCCATAC CCTGGGTTTT
1141     CCTCCAACCA TAAGGAAAAT TGATGCAGCT GTTTCTGACA AGGAAAAGAA GAAAACATAC
1201     TTCTTTGCAG CGGACAAATA CTGGAGATTT GATGAAAATA GCCAGTCCAT GGAGCAAGGC
1261     TTCCCTAGAC TAATAGCTGA TGACTTTCCA GGAGTTGAGC CTAAGGTTGA TGCTGTATTA
1321     CAGGCATTTG GATTTTTCTA CTTCTTCAGT GGATCATCAC AGTTTGAGTT TGACCCCAAT
1381     GCCAGGATGG TGACACACAT ATTAAAGAGT AACAGCTGGT TACATTGCTA G

Quality Control: cDNA sequencing results is available upon request.