MMP1 Protein (His Tag)

Matrix Metallopeptidase 1 ( MMP1 ) Protein

MMP1 Protein Price Inquiry ( Available Sizes )

50μg:  Inquiring Price;      200μg:  Inquiring Price;     ≥1mg Bulk:  Inquiring Price

MMP1 Protein Product Information

• MMP1 Synonym: CLG, CLGN
• Protein Construction: A DNA sequence encoding the pro form of human MMP1 enzyme ( NP_002412.1 ) precursor ( Met 1- Asn 469 ) was expressed with a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Human Cells

MMP1 Protein QC Testing

• Purity: > 96% as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2, AnaSpec Cat#27076
  The specific activity is > 70 pmoles / min / μg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 20
• Molecular Mass: The secreted recombinant human MMP1 comprises 461 amino acids migrates as an approximately 50-55 kDa band as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 25 mM MES, 10 mM CaCl₂, 150 mM NaCl, 0.05% Brij 35, pH 5.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MMP1 protein

MMP1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MMP1 Protein Description

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological and pathological processes such as morphogenesis, tissue remodeling, inflammation, as well as tumor invasion. MMPs are synthesized as enzymogen and converted to active forms by other MMPs or extracellular proteinases after removal of the propeptide. Human MMP1, also well known as interstitial collagenase, is an enzyme expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages, and can degrade a broad range of substrates, majorly types I, II, III, VII, VIII, and X collagens, as well as casein, gelatin, α-1 antitrypsin, etc. The mature form of MMP-1/MMP1 structurally consisits of a catalytic N-terminal domain and a C-terminal (hemopexin-like) domain involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). Additionally, studies indicated that MMP-1/MMP1 is also involved in the process of HIV infection. It interacts with and cleaves the HIV Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

References

1. Desrochers, P.E. et al., 1991, J. Clin. Invest. 87: 2258-2265.
2. Templeton, N.S. et al., 1990, Cancer. Res. 50: 5431-5437.
3. Nagase, H. et al., 1999, J. Biol. Chem. 31:21491-21494.
4. Rumbaugh, J. et al., 2006, FASEB. J. 20: 1736-1738.