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Rat CD10 / MME Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80288-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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MME cDNA Clone Datasheet
MME cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_012608.2 |
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cDNA Size: |
2253 |
| Gene Synonym: |
Nep; SFE; CD10; MGC93576; Mme |
| Source: | Rat |
MME cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus membrane metallo-endopeptidase DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 522 G/T resulting in the amino acid Trp substitution by Cys. |
MME cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
MME cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
MME cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGAAGAT CAGAAAGTCA GATGGATATT ACTGATATCA ATGCTCCAAA GCCGAAGAAG 61 AAACAGCGAT GGACTCCACT GGAGATCAGC CTTTCTGTGC TCGTCTTGCT CCTGACTATC 121 ATAGCTGTGA CAATGATTGC TCTCTATGCA ACCTATGATG ATGGTATTTG CAAATCATCA 181 GACTGCATAA AATCAGCTGC TCGACTGATC CAGAACATGG ATGCCTCTGC TGAGCCATGT 241 ACGGACTTCT TCAAATATGC TTGTGGAGGC TGGTTGAAAC GCAATGTCAT CCCTGAGACC 301 AGTTCCCGAT ACAGTAATTT TGACATTCTA AGAGATGAAC TAGAAGTCAT TTTGAAAGAT 361 GTCCTTCAAG AACCCAAAAC TGAGGACATA GTAGCAGTGC AGAAAGCAAA AACTTTGTAC 421 AGATCATGTA TAAATGAATC TGCTATTGAT AGCAGAGGTG GGCAACCTCT GCTCACACTG 481 TTACCAGATA TATATGGGTG GCCAGTAGCA TCACAAAACT GTGAACAAAC ATATGGTACT 541 TCTTGGACAG CTGAGAAATC TATTGCACAA CTGAATTCTA AATATGGGAA AAAGGTCCTC 601 ATTAATTTTT TTGTTGGCAC TGATGATAAG AATTCTACCC AGCATATAAT TCATTTTGAC 661 CAGCCTCGAC TTGGCCTCCC TTCCAGAGAC TACTATGAGT GTACAGGAAT ATATAAAGAG 721 GCTTGCACAG CATATGTGGA TTTTATGATT TCTGTGGCCA GACTGATTCG TCAGGAACAA 781 AGATTGCCTA TTGATGAAAA CCAGCTCTCT TTGGAAATGA ATAAAGTTAT GGAATTGGAA 841 AAAGAAATTG CCAATGCCAC AACTAAACCA GAAGACCGAA ATGACCCAAT GCTGCTTTAT 901 AACAAAATGA CATTGGCCAA GCTCCAAAAT AACTTCTCTC TGGAGATCAA TGGGAAGCCA 961 TTCAGCTGGT CAAATTTCAC AAATGAAATC ATGTCAACTG TGAATATTAA TATTCAAAAT 1021 GAGGAAGAAG TGGTTGTTTA TGCTCCAGAA TATTTAACCA AACTTAAGCC TATTCTTACC 1081 AAATATTCTC CCAGAGATCT TCAAAATTTA ATGTCCTGGA GGTTCATAAT GGATCTTGTA 1141 AGCAGCCTCA GCCGAAACTA CAAGGAGTCC AGAAATGCTT TCCGCAAGGC CCTTTACGGG 1201 ACTACATCCG AAACTGCAAC CTGGAGACGG TGTGCCAACT ACGTCAATGG GAACATGGAG 1261 AATGCTGTGG GGAGGCTTTA TGTGGAAGCA GCTTTTGCTG GAGAGAGCAA GCACGTGGTT 1321 GAAGATTTGA TTGCACAAAT CCGTGAAGTT TTTATTCAGA CTTTAGATGA CCTCACTTGG 1381 ATGGATGCTG AGACAAAAAA GAAAGCTGAA GAGAAGGCCC TGGCAATTAA AGAAAGGATT 1441 GGCTATCCTG ATGACATCAT CTCCAATGAG AATAAACTGA ATAATGAGTA TCTTGAGTTG 1501 AACTACAAGG AAGAGGAATA CTTTGAGAAC ATAATTCAAA ATTTGAAATT CAGCCAAAGC 1561 AAGCAGCTAA AGAAGCTCCG AGAAAAGGTG GACAAAGATG AGTGGATAAG TGGCGCGGCG 1621 GTAGTCAATG CATTTTATTC CTCAGGCAGA AATCAGATCG TCTTCCCCGC CGGCATTTTG 1681 CAGCCCCCAT TCTTTAGTGC TCGGCAGTCC AACTCATTGA ACTATGGGGG CATCGGCATG 1741 GTCATCGGAC ATGAAATCAC ACATGGCTTT GATGACAATG GCAGAAATTT TAACAAAGAT 1801 GGAGACCTCG TTGACTGGTG GACTCAGCAG TCTGCAAATA ATTTCAAAGA CCAATCCCAG 1861 TGTATGGTGT ACCAGTATGG AAACTTTACA TGGGACCTAG CAGGTGGACA GCATCTCAAT 1921 GGAATTAACA CACTAGGAGA AAATATTGCT GATAATGGAG GGATTGGCCA AGCATACAGA 1981 GCCTATCAGA ATTATGTTAA AAAGAATGGT GAAGAAAAAT TACTCCCTGG ACTTGACCTC 2041 AATCACAAAC AACTATTCTT CTTGAACTTT GCCCAGGTGT GGTGTGGAAC CTACCGGCCA 2101 GAGTATGCAG TCAATTCCAT TAAAACAGAT GTACACAGTC CTGGCAATTT CAGGATCATT 2161 GGGACTTTGC AGAACTCTGC TGAGTTTGCG GATGCCTTTC ATTGCCGCAA GAACTCATAC 2221 ATGAATCCAG AAAGGAAATG TCGGGTTTGG TGA
Quality Control:cDNA sequencing results is available upon request.
