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Human MKNK1 transcript variant 3 Gene cDNA Clone (full-length ORF Clone)

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    Human MKNK1 cDNA Clone Product Information
    NCBI RefSeq:NM_001135553.1
    RefSeq ORF Size:1275
    cDNA Description:ORF Clone of Homo sapiens MAP kinase interacting serine/threonine kinase 1, transcript variant 3 DNA.
    Gene Synonym:MKNK1, RP11-49P4.3
    Species:Human
    Vector:pMD18-T Simple Vector
    Plasmid:
    Restriction Site:
    Tag Sequence:
    Sequence Description:Same as Gene Bank Ref. ID sequence with insertion mutationthe 101bp after site 32, and the point mutation 108 C/T not causing the amino acid variation, the point mutation 952 G/A resulting in the amino acid ala substitution by thr.
    Sequencing primers:
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:
    Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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    Datasheet & Documentation

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