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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human MIF ORF mammalian expression plasmid, C-GFPSpark tag||HG10246-ACG|
|Human MIF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10246-ACR|
|Human MIF ORF mammalian expression plasmid, C-Flag tag||HG10246-CF|
|Human MIF ORF mammalian expression plasmid, C-His tag||HG10246-CH|
|Human MIF ORF mammalian expression plasmid, C-Myc tag||HG10246-CM|
|Human MIF ORF mammalian expression plasmid, C-HA tag||HG10246-CY|
|Human MIF ORF mammalian expression plasmid, N-Flag tag||HG10246-NF|
|Human MIF ORF mammalian expression plasmid, N-His tag||HG10246-NH|
|Human MIF ORF mammalian expression plasmid, N-Myc tag||HG10246-NM|
|Human MIF ORF mammalian expression plasmid, N-HA tag||HG10246-NY|
|Human MIF natural ORF mammalian expression plasmid||HG10246-UT|
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Macrophage migration inhibitory factor (MIF) is an immunoregulatory cytokine, the effect of which on arresting random immune cell movement was recognized several decades ago. Despite its historic name, MIF also has a direct chemokine-like function and promotes cell recruitment. MIF is an ubiquitously expressed protein that plays a crucial role in many inflammatory and autoimmune disorders. Increasing evidence suggests that MIF also controls metabolic and inflammatory processes underlying the development of metabolic pathologies associated with obesity. Further research has shown that MIF plays a particularly critical part in cell cycle regulation and therefore in tumorigenesis as well. The significance of the role of MIF in a variety of both solid and hematologic tumors has been established. More recently, interest has increased in the role of MIF in the development of central nervous system (CNS) tumors, in which it appears to influence cell cycle control. MIF contributes to malignant disease progression on several different levels. Both circulating and intracellular MIF protein levels are elevated in cancer patients and MIF expression reportedly correlates with stage, metastatic spread and disease-free survival. Blockade of MIF bioactivity successfully inhibited tumor cell growth in vivo and in vitro. MIF plays important roles in the pathogenesis of gastrointestinal, hepatic, and pancreatic disorders.