MICB / MIC-B Antibody ( FITC )

MICB / MIC-B Antibody Datasheet

MICB / MIC-B Antibody Product Information

• Immunogen: Recombinant Human MICB / MIC-B protein (Catalog#10759-H08H)
• Reagents: FITC-conjugated mouse monoclonal antibody
• Clone ID: 2F9E7
• Ig Type: Mouse IgG1
• Concentration: 5 μl/Test, 0.2 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MICB / MIC-B (rh MICB; Catalog#10759-H08H; NP_005922.2; Met 1-Gly 298) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

MICB / MIC-B Antibody Usage Guide

• Specificity: Human MICB / MIC-B
• Flow Cytometry: HeLa cells were detached using 1X trypsin, washed, then stained with FITC mouse anti-MICB.
  

  High Five cells were infected by Bac-NP (solid line histogram) or Bac-NA (dashed line histogram) , the cells were collected at 48 hours postinfection, and the levels of NP were measured by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human MICB in WB.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

MICB / MIC-B Antibody Background

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. Acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

References

1. Bauer, S. et al., 1999, Science. 285:727-729.
2. Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
3. Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
4. Stephens, H., 2001, Trends Immunol. 22: 378-385.
5. Borrego, F. et al., 2002, Mol. Immunol. 38: 637-660.
6. Groh, V. et al., 2002, Nature. 419:734-738.