After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

MICB / MIC-B Antibody, Mouse MAb

DatasheetReviewsRelated ProductsProtocols
Human MICB Antibody Product Information
Immunogen:Recombinant Human MICB protein (Catalog#10759-H08H)
Clone ID:12
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MICB (rh MICB; Catalog#10759-H08H; NP_005922.2; Met 1-Gly 298). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human MICB Antibody Usage Guide
Specificity:Human MICB / MIC-B
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human MICB. The detection limit for Human MICB is approximately 0.0195 ng/well .

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human MICB Antibody FC Application Image
MICB / MIC-B Antibody, Mouse MAb, Flow cytometric
[Click to enlarge image]
Flow cytometric analysis of anti-MICB (10759-MM12, 1 μg/test) on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified mouse anti-MICB. Second step staining with rabbit anti-mouse IgG FC polyclonal antibody.
Other MICB Antibody Products
MICB  Background

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

Human MICB  References
  • Bauer, S. et al., 1999, Science. 285:727-729.
  • Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
  • Stephens, H., 2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al., 2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al., 2002, Nature. 419:734-738.
  • Product nameProduct name
    Size / Price
    Catalog: 10759-MM12-50
    List Price: 
    Price:      (You Save: )
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.