Anti-MICB Antibody

Cat: 10759-MM12
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Anti-MICB Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-MICB Antibody
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human MICB
Immunogen
Recombinant Human MICB protein (Catalog#10759-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MICB (rh MICB; Catalog#10759-H08H; NP_005922.2; Met 1-Gly 298). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #12
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-MICB Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-MICB Antibody (Mouse Monoclonal antibody) Images
Human MICB  Flow Cytometry (FC) 5380
Flow cytometric analysis of anti-MICB (10759-MM12) on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified mouse anti-MICB. Second step staining with rabbit anti-mouse IgG FC polyclonal antibody.
Human MICB Flow Cytometry (FC) 19008
Flow cytometric analysis of anti-MICB (10759-MM12) on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified mouse anti-MICB. Second step staining with rabbit anti-mouse IgG FC polyclonal antibody.
Anti-MICB Antibody Alternative Names
Anti-PERB11.2 Antibody
MICB Background Information

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

Full Name
MHC class I polypeptide-related sequence B
References
  • Bauer, S. et al., 1999, Science. 285:727-729.
  • Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
  • Stephens, H., 2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al., 2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al., 2002, Nature. 419:734-738.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-MICB Antibody (APC) Mouse Monoclonal FCM 10759-MM12-A
    Anti-MICB Antibody Rabbit Polyclonal WB,ELISA 10759-T48
    Anti-MICB Antibody Rabbit Monoclonal WB 10759-R102
    Anti-MICB Antibody Rabbit Polyclonal ELISA,IHC-P 10759-RP02
    Anti-MICB Antibody Rabbit Polyclonal WB,IHC-P 10759-T40
    Anti-MICB Antibody Rabbit Polyclonal ELISA 10759-RP01
    Anti-MICB Antibody (FITC) Mouse Monoclonal FCM 10759-MM12-F
    Anti-MICB Antibody (PE) Mouse Monoclonal FCM 10759-MM12-P
    Anti-MICB Antibody Rabbit Monoclonal ELISA 10759-R103
    Anti-MICB Antibody Mouse Monoclonal ELISA(Cap) 10759-MM04
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