|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
MICA was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-MICA rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-MICA rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 55 kDa
Observed band size: 55 kDa
Anti-MICA rabbit polyclonal antibody at 1:500 dilution
Lane A: 293T Whole Cell Lysate
Lane B: Jurkat Whole Cell Lysate
Lane C: Daudi Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:43 kDa
Observed band size:55 kDa
MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-MICA Antibody (APC)||Mouse||Monoclonal||FCM||12302-MM04-A|
|Anti-MICA Antibody (FITC)||Mouse||Monoclonal||FCM||12302-MM04-F|
|Anti-MICA Antibody (PE)||Mouse||Monoclonal||FCM||12302-MM04-P|