Human MIA Gene cDNA Clone / ORF Clone

MIA cDNA Clone Datasheet

MIA cDNA Clone Product Information

• Gene Bank Ref.ID: BC005910
• cDNA Size: 396
• Gene Synonym: CD-RAP; MIA
• Source: Human

MIA cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens melanoma inhibitory activity DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MIA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MIA cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MIA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCGGT CCCTGGTGTG CCTTGGTGTC ATCATCTTGC TGTCTGCCTT CTCCGGACCT
61       GGTGTCAGGG GTGGTCCTAT GCCCAAGCTG GCTGACCGGA AGCTGTGTGC GGACCAGGAG
121      TGCAGCCACC CTATCTCCAT GGCTGTGGCC CTTCAGGACT ACATGGCCCC CGACTGCCGA
181      TTCCTGACCA TTCACCGGGG CCAAGTGGTG TATGTCTTCT CCAAGCTGAA GGGCCGTGGG
241      CGGCTCTTCT GGGGAGGCAG CGTTCAGGGA GATTACTATG GAGATCTGGC TGCTCGCCTG
301      GGCTATTTCC CCAGTAGCAT TGTCCGAGAG GACCAGACCC TGAAACCTGG CAAAGTCGAT
361      GTGAAGACAG ACAAATGGGA TTTCTACTGC CAGTGA

 

Quality Control:cDNA sequencing results is available upon request.