Mouse MFI2 Gene cDNA Clone / ORF Clone

MFI2 cDNA Clone Datasheet

MFI2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013900.2
• cDNA Size: 2217
• Gene Synonym: MTf; CD228; Mfi2
• Source: Mouse

MFI2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 915 T/C not causing the amino acid variation.

MFI2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MFI2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MFI2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCTCC TGAGCGTGAC TTTTTGGCTA CTCCTGTCCC TGCGCACTGT CGTCTGTGTG
61       ATGGAGGTGC AGTGGTGTAC CATCTCAGAC GCAGAGCAGC AGAAGTGCAA AGACATGAGC
121      GAGGCCTTCC AGGGAGCTGG CATTCGTCCT TCCCTTCTCT GCGTCCAGGG CAACTCCGCT
181      GACCACTGTG TCCAGCTCAT CAAGGAACAA AAAGCAGATG CCATCACCCT GGATGGAGGG
241      GCCATCTATG AGGCAGGGAA GGAGCACGGC CTGAAGCCAG TGGTGGGGGA AGTCTATGAC
301      CAAGACATTG GGACTTCCTA TTATGCCGTG GCTGTGGTCA GGAGGAATTC CAATGTTACC
361      ATCAACACCC TGAAGGGCGT CAAGTCCTGC CACACAGGCA TTAACCGGAC TGTGGGCTGG
421      AACGTGCCTG TCGGTTACCT CGTAGAGAGC GGCCATCTGT CAGTGATGGG CTGTGATGTG
481      CTCAAAGCCG TTGGTGATTA TTTTGGAGGC AGCTGTGTCC CTGGAACAGG AGAAACCAGC
541      CATTCCGAGT CCCTCTGTCG CCTCTGCCGT GGCGACTCTT CTGGGCACAA TGTGTGTGAC
601      AAGAGTCCCC TAGAGAGATA CTACGACTAC AGTGGAGCCT TCCGGTGCCT GGCGGAAGGA
661      GCCGGTGACG TGGCCTTCGT GAAGCACAGC ACAGTGCTGG AAAATACTGA TGGAAACACC
721      CTGCCTTCCT GGGGCAAGTC CCTGATGTCA GAGGACTTCC AGCTACTATG CAGGGATGGC
781      AGCCGAGCCG ACATCACTGA GTGGAGACGT TGCCACCTGG CCAAGGTGCC TGCTCATGCT
841      GTGGTGGTCA GGGGTGACAT GGATGGCGGT CTCATATTCC AACTGCTCAA CGAAGGCCAG
901      CTTCTGTTCA GCCACGAAGA CAGCAGCTTC CAGATGTTCA GCTCCAAAGC CTACAGCCAG
961      AAGAACTTGC TGTTCAAAGA CTCCACCTTG GAGCTTGTGC CCATTGCCAC ACAGAACTAT
1021     GAGGCCTGGC TGGGCCAGGA ATACCTGCAG GCCATGAAGG GGCTCCTCTG TGATCCCAAC
1081     CGGCTGCCCC ACTACCTGCG CTGGTGTGTG CTGTCAGCGC CCGAGATCCA GAAGTGTGGA
1141     GATATGGCTG TGGCCTTCAG CCGCCAGAAT CTCAAGCCGG AAATTCAGTG TGTGTCGGCC
1201     GAGTCCCCTG AGCACTGCAT GGAGCAGATC CAGGCTGGGC ACACTGACGC TGTGACTCTG
1261     AGGGGCGAGG ACATTTACAG GGCAGGAAAG GTGTACGGCC TGGTTCCGGC GGCCGGGGAG
1321     CTGTATGCTG AGGAGGACAG GAGCAATTCC TACTTTGTGG TGGCTGTGGC AAGAAGGGAC
1381     AGCTCCTACT CCTTCACCCT GGACGAGCTT CGCGGCAAGC GTTCCTGCCA CCCCTACTTG
1441     GGCAGCCCAG CGGGCTGGGA GGTGCCCATC GGCTCCCTCA TCCAGCGGGG CTTCATCCGG
1501     CCCAAGGACT GTGATGTCCT CACAGCGGTG AGCCAGTTCT TCAATGCCAG CTGCGTGCCT
1561     GTCAACAACC CTAAGAACTA CCCTTCCGCA CTATGTGCGC TCTGCGTGGG AGACGAGAAG
1621     GGCCGCAACA AATGTGTGGG GAGCAGCCAG GAGAGATACT ACGGCTACAG CGGGGCCTTC
1681     AGGTGCCTTG TGGAGCATGC AGGGGACGTG GCTTTCGTCA AGCACACGAC TGTCTTTGAG
1741     AACACAAATG GTCACAATCC TGAGCCTTGG GCTTCTCACC TCAGGTGGCA AGACTATGAA
1801     CTACTGTGCC CCAATGGGGC ACGGGCTGAG GTAGACCAGT TCCAAGCTTG CAACCTGGCA
1861     CAAATGCCAT CCCACGCTGT CATGGTCCGT CCAGACACCA ACATCTTCAC TGTGTATGGA
1921     CTTCTGGACA AGGCCCAGGA CCTGTTTGGA GACGACCATA ACAAGAACGG TTTCCAAATG
1981     TTTGACTCCT CCAAATATCA CAGCCAAGAC CTGCTTTTCA AAGATGCTAC AGTCCGAGCG
2041     GTGCCAGTCC GGGAGAAAAC CACATACCTG GACTGGCTGG GTCCTGACTA TGTGGTTGCG
2101     CTGGAGGGGA TGTTGTCTCA GCAGTGCTCC GGTGCAGGGG CCGCGGTGCA GCGAGTCCCC
2161     CTGCTGGCCC TGCTCCTGCT GACCCTGGCT GCAGGCCTCC TTCCTCGCGT TCTCTGA

 

Quality Control:cDNA sequencing results is available upon request.