Mouse MFI2 / CD228 / melanotransferrin Protein

Antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 Protein Datasheet

MFI2 / CD228 / melanotransferrin Protein Product Information

• Synonym: CD228, MTf
• Protein Construction: A DNA sequence encoding the mouse MFI2 (Q9R0R1) (Met 1-Gln 708), without the propeptide, was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

MFI2 / CD228 / melanotransferrin Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 20
• Molecular Mass: The secreted recombinant mouse MFI2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 930 amino acids and has a calculated molecular mass of 103 kDa. The apparent molecular mass of rmMFI2/Fc monomer is approximately 100 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MFI2 / CD228 / melanotransferrin protein

MFI2 / CD228 / melanotransferrin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MFI2 / CD228 / melanotransferrin Protein Description

The melanotransferrin (MTf) molecule, also called CD228 or p97, has been widely described as a melanoma specific molecule. MTf is found at high levels in melanoma cells and previous studies have shown that MTf can bind Fe. MTf has a high level of sequence homology to transferrin (Tf) and lactoferrin, but is unusual because it predominantly occurs as a membrane bound, glycosylphosphatidylinositol (GPI) anchored molecule, but can also occur as a soluble form. The iron-binding properties of melanotransferrin, the tumour-associated antigen p97, have been investigated by many aspects and the results show that, in contrast to other transferrins, melanotransferrin binds only one Fe³⁺ ion per molecule. The binding properties of its N-terminal site are similar to other transferrins, but its C-terminal site does not bind iron at all. The differences can be related to specific amino acid changes in the C-terminal site. In clinical, The protein melanotransferrin (MTf) is associated with the brain lesions of Alzheimer's disease (AD) and is a potential marker of the disorder. Serum MTf is a candidate marker of AD, even in the early stage when clinical diagnosis is most uncertain.

References

1. Richardson DR. 2000, Eur J Biochem. 267 (5): 1290-8.
2. Kim DK. et al., 2001, Neuropsychopharmacology. 25 (1): 84-90.
3. Rothenberger S. et al., 1996, Brain Res. 712 (1): 117-21.
4. Baker EN. et al., 1992, FEBS Lett. 298 (2-3): 215-8.