MFG-E8 / lactadherin / MFGE8 Protein (His Tag)

Milk fat globule-EGF factor 8 protein Protein Datasheet

MFG-E8 / lactadherin / MFGE8 Protein Product Information

• Synonym: BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47
• Protein Construction: A DNA sequence encoding the human MFGE8 isoform 1(Q08431-1) (Met 1- Cys 387) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

MFG-E8 / lactadherin / MFGE8 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 24
• Molecular Mass: The secreted recombinant human MFGE8 comprises 374 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh MFGE8 is approximately 45 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MFG-E8 / lactadherin / MFGE8 protein

MFG-E8 / lactadherin / MFGE8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MFG-E8 / lactadherin / MFGE8 Protein Description

MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. Opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells, mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.

References

1. Oshima K. et al., 2002, Eur J Biochem. 269 (4): 1209-18.
2. Hanayama R. et al., 2002, Nature. 417 (6885): 182-7.
3. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903.