Anti-METTL1 Antibody

Cat: 11525-MM05
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Anti-METTL1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-METTL1 Antibody
Validated applications
WB,ELISA,ELISA(Det),IP
Species reactivity
Reacts with: Human
Specificity
Human METTL1
No cross-reactivity in ELISA with E.coli cell lysate
Immunogen
Recombinant Human METTL1 protein (Catalog#11525-H07E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (rh METTL1; Catalog#11525-H07E; NP_005362.3; Asp 32-Gln 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9C2F2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-METTL1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 4-6 μL/mg of lysate  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human METTL1 in ELISA pair set (Catalog: # SEK11525). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11525-MM02).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-METTL1 Antibody (Mouse Monoclonal antibody) Images
Human ABHD14B Immunoprecipitation(IP) 8176

METTL1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-METTL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-METTL1 mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 34 kDa

Observed band size: 34 kDa

Human METTL1 Western blot (WB) 13409

Anti-METTL1 mouse monoclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:31 kDa

Anti-METTL1 Antibody Alternative Names
Anti-C12orf1 Antibody;Anti-TRM8 Antibody;Anti-TRMT8 Antibody;Anti-YDL201w Antibody
METTL1 Background Information

tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein which belongs to the methyltransferase superfamily and TrmB family. METTL1 gene, has been identified by its sequence similarity to the yeast ORF YDL201w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL201w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.

Full Name
methyltransferase like 1
References
  • Bahr, A.et al., 1999, Genomics. 57 (3):424-8.
  • Wikman, H. et al., 2005,Genes Chromosomes Cancer. 42 (2):193-9.
  • Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-METTL1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 11525-RP02
    Anti-METTL1 Antibody Rabbit Polyclonal ELISA 11525-RP01
    Anti-METTL1 Antibody Mouse Monoclonal ELISA(Cap) 11525-MM02
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