Human METRNL Gene cDNA Clone / ORF Clone

Human METRNL cDNA Clone Datasheet

METRNL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001004431.1
• cDNA Size: 936
• Gene Synonym: MGC99788; METRNL
• Source: Human

METRNL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens meteorin, glial cell differentiation regulator-like DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 21 G/A and 24 C/A not causing the amino acid variation.

METRNL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

METRNL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

METRNL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGGGCG CGGCGCGGGC AGCATGGGGG CGCGCGGGGC AGCCGTGGCC GCGACCCCCC
61       GCCCCGGGCC CGCCCCCGCC GCCGCTCCCG CTGCTGCTCC TGCTCCTGGC CGGGCTGCTG
121      GGCGGCGCGG GCGCGCAGTA CTCCAGCGAC CGGTGCAGCT GGAAGGGGAG CGGGCTGACG
181      CACGAGGCAC ACAGGAAGGA GGTGGAGCAG GTGTATCTGC GCTGTGCGGC GGGTGCCGTG
241      GAGTGGATGT ACCCAACAGG TGCTCTCATC GTTAACCTGC GGCCCAACAC CTTCTCGCCT
301      GCCCGGCACC TGACCGTGTG CATCAGGTCC TTCACGGACT CCTCGGGGGC CAATATTTAT
361      TTGGAAAAAA CTGGAGAACT GAGACTGCTG GTACCGGACG GGGACGGCAG GCCCGGCCGG
421      GTGCAGTGTT TTGGCCTGGA GCAGGGCGGC CTGTTCGTGG AGGCCACGCC GCAGCAGGAT
481      ATCGGCCGGA GGACCACAGG CTTCCAGTAC GAGCTGGTTA GGAGGCACAG GGCGTCGGAC
541      CTGCACGAGC TGTCTGCGCC GTGCCGTCCC TGCAGTGACA CCGAGGTGCT CCTAGCCGTC
601      TGCACCAGCG ACTTCGCCGT TCGAGGCTCC ATCCAGCAAG TTACCCACGA GCCTGAGCGG
661      CAGGACTCAG CCATCCACCT GCGCGTGAGC AGACTCTATC GGCAGAAAAG CAGGGTCTTC
721      GAGCCGGTGC CCGAGGGTGA CGGCCACTGG CAGGGGCGCG TCAGGACGCT GCTGGAGTGT
781      GGCGTGCGGC CGGGGCATGG CGACTTCCTC TTCACTGGCC ACATGCACTT CGGGGAGGCG
841      CGGCTCGGCT GTGCCCCACG CTTCAAGGAC TTCCAGAGGA TGTACAGGGA TGCCCAGGAG
901      AGGGGGCTGA ACCCTTGTGA GGTTGGCACG GACTGA

 

Quality Control:cDNA sequencing results is available upon request.