Mouse METAP2 / MAP2 Protein (His Tag)

Methionine aminopeptidase 2 Protein Datasheet

METAP2 / MAP2 Protein Product Information

• Synonym: 4930584B20Rik, A930035J23Rik, AI047573, AL024412, AU014659, Amp2, Mnpep, p67, p67eIF2
• Protein Construction: A DNA sequence encoding the mouse METAP2 (O08663) (Ala 2-Tyr 478) was expressed，with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

METAP2 / MAP2 Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse METAP2 consists of 488 amino acids and has a calculated molecular mass of 54.3 kDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris,100mM NaCl, pH 8.0, 10%glycerol
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: METAP2 / MAP2 protein

METAP2 / MAP2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

METAP2 / MAP2 Protein Description

METAP2(Methionine aminopeptidase 2,Histidine-tagged), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. Furthermore, MAP2 is of particular interest because the enzyme plays a key role in angiogenesis, the growth of new blood vessels, which is necessary for the progression of diseases including solid tumor cancers and rheumatoid arthritis. Researchers have also found that MetAP2 is the target of two groups of anti-angiogenic natural products, ovalicin and fumagillin, and their analogs. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein.

References

1. Bennett. et al., 1997, J Am Chem Soc. 119: 1923-33.
2. Johansson. et al., 2008, Inorg Chem. 47: 5079-92.
3. Bradshaw. et al., 2002, Essays Biochem. 38: 65-78.