Mouse METAP1D Gene cDNA Clone / ORF Clone

METAP1D cDNA Clone Datasheet

METAP1D cDNA Clone Product Information

• Gene Bank Ref.ID: NM_025633.3
• cDNA Size: 1008
• Gene Synonym: Map1d; Metapl1; AV117938; 2310066F24Rik; 3110033D18Rik; Metap1d
• Source: Mouse

METAP1D cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus methionyl aminopeptidase type 1D (mitochondrial) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

METAP1D cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

METAP1D cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

METAP1D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCGC CCATCGGCGT CCCCCTGCTC GTCCGTGGAG GTTGTCAGAG AATTCTGTCT
61       TCGCCACTCA ATCATATCTA CTTACACAAG CGGTCAGGCA GTCAACAAAG AAGACATTTC
121      TTTTTCTGGA GACAAAGGGA TATCTCCCAC AGTGTGGTTT CACCGGCTGC CGTCTCTCCC
181      GCACATCCTG TCCCCAAGCG CATAAAGAAG CCAGACTACG TGACGACAGG CATTGTGCCG
241      GACTGGGGAG ACAGCATAGA AGTGAAGGAT GAAGATCAGA TTCAAGGGCT TCGTGAGGCT
301      TGTCGGCTGG CGAGACACGT CCTCCTCCTG GCTGGGAAGA GTTTAAAGGT TGACATGACA
361      ACTGAAGAGA TAGATGCGCT TGTTCATTGG GAAATCATCA GGCATGACGC ATATCCCTCA
421      CCTCTGGGCT ATGGACGCTT TCCAAAATCT GTTTGCACCT CTGTAAACAA CGTGCTCTGT
481      CATGGCATTC CTGACAGCCG ACCTCTTCAG GATGGAGATA TTATCAACAT TGATGTCACG
541      GTCTATTACA ATGGCTACCA TGGAGACACC TCTGAAACCT TTCTGGTGGG CAATGTGGAT
601      GAAAGTGGTA AAAAGTTAGT GGAGGTTGCC AGGAGGTGTA GAGATGAAGC GATTGCAGCC
661      TGCAGAGCTG GGGCCCCCTT CTCTGTAATT GGAAACACAA TCAGCCGCAT AACTCATCAG
721      AATGGTTTGC AAGTCTGTCC ACATTTTGTG GGACATGGGA TAGGATCATA CTTTCATGGA
781      CACCCAGAAA TTTGGCATCA TGCAAATGAC AATGATCTGC CCATGGAGGA GGGCATGGCT
841      TTCACCATAG AGCCAATCAT CACTGAAGGT TCCCCAGAAT TTAAAGTCCT TGAGGACGCA
901      TGGACCGTGG TCTCCCTAGA CAACCAAAGG TCTGCGCAGT TCGAGCACAC AGTCCTCATC
961      ACGCCAAGGG GAGTGGAGAT ACTGACCAAA CTGCCACAAG AAGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.