Mouse MESDC2 Gene cDNA Clone / ORF Clone

MESDC2 cDNA Clone Datasheet

MESDC2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_023403.2
• cDNA Size: 674
• Gene Synonym: AW537813; MGC25959; mKIAA0081; 2210015O11Rik; Mesdc2
• Source: Mouse

MESDC2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mesoderm development candidate 2 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 156 C/G not causing the amino acid variation.

MESDC2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MESDC2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

MESDC2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCCT CCAGGTGGCT GCGCGCGGTT CTACTGTTTC TGTGTGCCTC GGACCTTCTG
61       TTGCTCCCTC CTCCTAATGC CTACGCGGCG GACACTCCGG GCGAGGCCAC CCCACCTCCA
121      CGGAAAAAGA AGGACATCCG CGATTACAAC GATGCGGACA TGGCGCGACT TCTGGAGCAG
181      TGGGAGAAAG ATGATGACAT AGAAGAAGGA GACCTTCCAG AACACAAGAG ACCCTCAGCA
241      CCTATCGACT TCTCAAAGCT AGACCCAGGC AAACCTGAGA GCATCTTGAA AATGACAAAG
301      AAAGGGAAGA CTCTGATGAT GTTTGTCACC GTGTCTGGGA ACCCCACCGA GAAGGAGACA
361      GAGGAGATCA CCAGCCTGTG GCAGGGTAGC CTGTTCAACG CCAACTATGA CGTTCAGAGG
421      TTCATCGTGG GATCCGACCG CGCCATCTTC ATGCTCCGGG ATGGGAGCTA TGCCTGGGAG
481      ATCAAGGACT TTTTGGTCAG TCAAGACCGG TGTGCTGAAG TCACTCTAGA GGGACAGATG
541      TATCCTGGCA AAGGAGGAGG AAGCAAGGAG AAAAATAAAA CAAAGCCAGA GAAGGCTAAA
601      AAGAAGGAGG GAGATCCCAA ACCACGTGCT TCCAAGGAAG ACAATCGAGC TGGGAGCAGA
661      AGAGAAGACC TTTA

 

Quality Control:cDNA sequencing results is available upon request.