MESDC2 / MESD Protein (His Tag)

Mesoderm Development Candidate 2 ( MESDC2 / MESD ) Protein

MESDC2 / MESD Protein Product Information

• Synonym: UNQ1911/PRO4369, BOCA, KIAA0081, MESD, MESDC2
• Protein Construction: A DNA sequence encoding the human MESDC2 ( NP_055969.1 ) ( Ala 34 - Lys 230 ) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

MESDC2 / MESD Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 34
• Molecular Mass: The recombinant human MESDC2 consists of 208 amino acids and has a predicted molecular mass of 23.6 kDa. The apparent molecular mass of rh MESDC2 is approximately 27 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MESDC2 protein

MESDC2 / MESD Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MESDC2 / MESD Protein Description

LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61 and MESDC2, is a member of the MESD family. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N- and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway, since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for specification of embryonic polarity and mesoderm induction.

References

1. Scanlan M.J. et al., 1999, Int. J. Cancer 83:456-464.
2. Veltman,IM. et al.,2005, Hum Mol Genet  14 (14):1955-63.
3. Koduri V. et al., 2007, Biochemistry 46:6570-7.