Anti-MESDC2 Antibody

Cat: 10949-RP02
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Anti-MESDC2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-MESDC2 Antibody
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human MESDC2
Immunogen
Recombinant Human MESD protein (Catalog#10949-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human MESDC2 (rh MESDC2; Catalog#10949-H08H; NP_055969.1; Ala 34-Lys 230). MESDC2 specific IgG was purified by human MESDC2 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-MESDC2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-MESDC2 Antibody (Rabbit Polyclonal antibody) Images
Human MESDC2 Immunoprecipitation(IP) 8034

MESDC2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg NIH-3T3 Whole Cell Lysate

2 µL anti-MESDC2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MESDC2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 26 kDa

Observed band size: 26 kDa

Human MESDC2 Western blot (WB) 13308

Anti-MESDC2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:25 kDa

(We are unsure as to the identity of these extra bands.)

Anti-MESDC2 Antibody Alternative Names
Anti-BOCA Antibody;Anti-MESD Antibody
MESDC2 Background Information

LDLR chaperone MESD, also known as Mesoderm development protein, Mesoderm development candidate 2, Renal carcinoma antigen NY-REN-61 and MESDC2, is a member of the MESD family. MESDC2 is a chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). The LDLR maturation activity resides in the N- and C-terminal unstructured regions. MESDC2 acts as a modulator of the Wnt pathway, since some LDLRs are coreceptors for the canonical Wnt pathway. MESDC2 is essential for specification of embryonic polarity and mesoderm induction.

Full Name
mesoderm development candidate 2
References
  • Scanlan M.J. et al., 1999, Int. J. Cancer 83:456-464.
  • Veltman,IM. et al.,2005, Hum Mol Genet  14 (14):1955-63.
  • Koduri V. et al., 2007, Biochemistry 46:6570-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-MESDC2 Antibody Rabbit Polyclonal ELISA 10949-RP01
    Anti-MESDC2 Antibody Rabbit Monoclonal WB,ELISA,IP 10949-R502
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