Meprin alpha Matched ELISA Antibody Pair Set,Mouse General Information
Meprin alpha Matched ELISA Antibody Pair Set,Mouse
Solid Phase Sandwich ELISA
Quantitative determination of Mouse Meprin alpha
1. Capture Antibody 1.0 mg/mL of rabbit anti-mouse MEP1A monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL rabbit anti-mouse MEP1A polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 170 ng of recombinant mouse MEP1A. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
This Meprin alpha Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse Meprin alpha . It contains Mouse Meprin alpha capture antibody, Mouse Meprin alpha detector antibody
and a highly purified
recombinant Mouse Meprin alpha protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Meprin alpha Matched ELISA Antibody Pair Set,Mouse Images
Meprin alpha Matched ELISA Antibody Pair Set,Mouse Alternative Names
AI098089 Matched ELISA Antibody Pair Set, Mouse;AW107200 Matched ELISA Antibody Pair Set, Mouse;Mep-1 Matched ELISA Antibody Pair Set, Mouse;Mep-1a Matched ELISA Antibody Pair Set, Mouse;Mep1 Matched ELISA Antibody Pair Set, Mouse
Meprin alpha Background Information
Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).
meprin A, alpha (PABA peptide hydrolase)
Bertenshaw,GP. et al., 2002, Biol Chem. 383 (7-8):1175-83. Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22. Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18. Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.