MEP1A Antibody

MEP1A Antibody Datasheet

MEP1A Antibody Product Information

• Immunogen: Recombinant Mouse MEP1A Protein (Catalog#50057-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse MEP1A (rM MEP1A; Catalog#50057-M08H; NP_032611.2; Met 1 - Arg 615).

MEP1A Antibody Usage Guide

• Specificity: Mouse MEP1A
• Immunochemistry: IHC-P: 1-10 μg/mL

  

  Immunochemical staining of mouse MEP1A in mouse intestine with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of mouse MEP1A in mouse kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse MEP1A in WB. Using a DAB detection system, the detection limit for Mouse MEP1A is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse MEP1A. The detection limit for Mouse MEP1A is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

MEP1A Antibody Background

Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I  membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).

References

1. Bertenshaw,GP. et al., 2002, Biol Chem. 383 (7-8):1175-83.
2. Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22.
3. Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18.
4. Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.