Anti-Meprin alpha Antibody

Cat: 50057-RP01
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Anti-Meprin alpha Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Meprin alpha Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse Meprin alpha
Immunogen
Recombinant Mouse MEP1A protein (Catalog#50057-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse MEP1A (rM MEP1A; Catalog#50057-M08H; NP_032611.2; Met 1-Arg 615). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Meprin alpha Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Meprin alpha Antibody (Rabbit Polyclonal antibody) Images
Mouse MEP1A Western blot (WB) 13638

Anti-MEP1A rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:84 kDa

Observed band size:70 kDa

Anti-Meprin alpha Antibody Alternative Names
Anti-AI098089 Antibody;Anti-AW107200 Antibody;Anti-Mep-1 Antibody;Anti-Mep-1a Antibody;Anti-Mep1 Antibody
Meprin alpha Background Information

Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I  membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).

Full Name
meprin A, alpha (PABA peptide hydrolase)
References
  • Bertenshaw,GP. et al., 2002, Biol Chem. 383 (7-8):1175-83.
  • Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22.
  • Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18.
  • Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Meprin alpha Antibody Rabbit Polyclonal WB,ELISA 50057-RP02
    Anti-Meprin alpha Antibody Rabbit Monoclonal ELISA,IHC-P 50057-R001
    Anti-Meprin alpha Antibody Rabbit Monoclonal ELISA 10133-R009
    Anti-Meprin alpha Antibody Rabbit Polyclonal ELISA 10133-RP01
    Anti-Meprin alpha Antibody Rabbit Monoclonal ELISA 10133-R017
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