Human MED1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13221-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

MED1 cDNA Clone Datasheet

MED1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_004774.3
cDNA Size:	1671
Gene Synonym:	PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220; MGC71488；MED1
Source:	Human

MED1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens mediator complex subunit 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

MED1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MED1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MED1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAGCTC AGGGGGAAAC CGAGGAGTCA GAAAAGCTGA GTAAGATGAG TTCTCTCCTG
61       GAACGGCTCC ATGCAAAATT TAACCAAAAT AGACCCTGGA GTGAAACCAT TAAGCTTGTG
121      CGTCAAGTCA TGGAGAAGAG GGTTGTGATG AGTTCTGGAG GGCATCAACA TTTGGTCAGC
181      TGTTTGGAGA CATTGCAGAA GGCTCTCAAA GTAACATCTT TACCAGCAAT GACTGATCGT
241      TTGGAGTCCA TAGCAAGACA GAATGGACTG GGCTCTCATC TCAGTGCCAG TGGCACTGAA
301      TGTTACATCA CGTCAGATAT GTTCTATGTG GAAGTGCAGT TAGATCCTGC AGGACAGCTT
361      TGTGATGTAA AAGTGGCTCA CCATGGGGAG AATCCTGTGA GCTGTCCGGA GCTTGTACAG
421      CAGCTAAGGG AAAAAAATTT TGATGAATTT TCTAAGCACC TTAAGGGCCT TGTTAATCTG
481      TATAACCTTC CAGGGGACAA CAAACTGAAG ACTAAAATGT ACTTGGCTCT CCAATCCTTA
541      GAACAAGATC TTTCTAAAAT GGCAATTATG TACTGGAAAG CAACTAATGC TGGTCCCTTG
601      GATAAGATTC TTCATGGAAG TGTTGGCTAT CTCACACCAA GGAGTGGGGG TCATTTAATG
661      AACCTGAAGT ACTATGTCTC TCCTTCTGAC CTACTGGATG ACAAGACTGC ATCTCCCATC
721      ATTTTGCATG AGAATAATGT TTCTCGATCT TTGGGCATGA ATGCATCAGT GACAATTGAA
781      GGAACATCTG CTGTGTACAA ACTCCCAATT GCACCATTAA TTATGGGGTC ACATCCAGTT
841      GACAATAAAT GGACCCCTTC CTTCTCCTCA ATCACCAGTG CCAACAGTGT TGATCTTCCT
901      GCCTGTTTCT TCTTGAAATT TCCCCAGCCA ATCCCAGTAT CTAGAGCATT TGTTCAGAAA
961      CTGCAGAACT GCACAGGAAT TCCATTGTTT GAAACTCAAC CAACTTATGC ACCCCTGTAT
1021     GAACTGATCA CTCAGTTTGA GCTATCAAAG GACCCTGACC CCATACCTTT GAATCACAAC
1081     ATGAGATTTT ATGCTGCTCT TCCTGGTCAG CAGCACTGCT ATTTCCTCAA CAAGGATGCT
1141     CCTCTTCCAG ATGGCCGAAG TCTACAGGGA ACCCTTGTTA GCAAAATCAC CTTTCAGCAC
1201     CCTGGCCGAG TTCCTCTTAT CCTAAATCTG ATCAGACACC AAGTGGCCTA TAACACCCTC
1261     ATTGGAAGCT GTGTCAAAAG AACTATTCTG AAAGAAGATT CTCCTGGGCT TCTCCAATTT
1321     GAAGTGTGTC CTCTCTCAGA GTCTCGTTTC AGCGTATCTT TTCAGCACCC TGTGAATGAC
1381     TCCCTGGTGT GTGTGGTAAT GGATGTGCAG GACTCAACAC ATGTGAGCTG TAAACTCTAC
1441     AAAGGGCTGT CGGATGCACT GATCTGCACA GATGACTTCA TTGCCAAAGT TGTTCAAAGA
1501     TGTATGTCCA TCCCTGTGAC GATGAGGGCT ATTCGGAGGA AAGCTGAAAC CATTCAAGCC
1561     GACACCCCAG CACTGTCCCT CATTGCAGAG ACAGTTGAAG ACATGGTGAA AAAGAACCTG
1621     CCCCCGGCTA GCAGCCCAGG ATCAAAGAAT CCCGAGTTAG GATCTGGATG A

Quality Control:cDNA sequencing results is available upon request.