+86-400-890-9989
Product Catalog
Research Topics
Rat MDH1 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80232-G |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
MDH1 cDNA Clone Datasheet
MDH1 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_033235.1 |
|
cDNA Size: |
1005 |
| Gene Synonym: |
MDL1; Mdhl; Mor2;Mdh1 |
| Source: | Rat |
MDH1 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Rattus norvegicus malate dehydrogenase 1, NAD (soluble) DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref.ID sequence. |
MDH1 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
MDH1 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
MDH1 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGTCTGAGC CAATCAGAGT CCTCGTGACA GGAGCAGCCG GTCAGATTGC ATATTCGCTG 61 CTGTACAGCA TTGGAAATGG ATCTGTCTTT GGGAAAGACC AGCCCATCAT TCTTGTGCTG 121 TTGGACATCA CCCCCATGAT GGGTGTTCTG GACGGTGTCC TGATGGAGCT GCAAGACTGT 181 GCCCTTCCCC TTCTGCAGGA TGTCATTGCA ACAGACAAAG AAGAGGTTGC CTTCAAAGAC 241 CTGGACGTGG CTGTCCTTGT GGGCTCCATG CCAAGAAGGG AGGGCATGGA GAGGAAGGAC 301 CTACTGAAAG CCAACGTGAA GATCTTCAAA TCCCAGGGCG CAGCCTTGGA GAAGTACGCC 361 AAGAAATCAG TTAAGGTCAT TGTTGTGGGG AACCCAGCCA ATACAAACTG CCTGACGGCC 421 TCCAAGTCAG CACCATCGAT CCCCAAGGAG AACTTCAGTT GCCTGACTCG ATTGGACCAC 481 AACCGAGCAA AATCTCAAAT TGCTCTTAAA CTCGGTGTAA CCGCTGATGA TGTAAAAAAT 541 GTCATTATCT GGGGAAATCA TTCATCAACC CAGTATCCAG ATGTCAATCA TGCCAAGGTG 601 AAATTGCAAG GAAAAGAAGT TGGTGTGTAT GAAGCCCTCA AAGACGACAG CTGGCTCAAG 661 GGAGAGTTCA TCACGACTGT GCAGCAGCGT GGTGCTGCTG TCATCAAGGC TCGGAAGCTG 721 TCCAGTGCCA TGTCTGCTGC GAAGGCCATC TCGGACCACA TCAGAGACAT CTGGTTTGGA 781 ACCCCGGAGG GCGAGTTCGT GTCGATGGGC GTAATCTCTG ATGGCAACTC CTATGGTGTC 841 CCTGATGACC TGCTCTACTC GTTCCCTGTC GTGATCAAGA ATAAGACCTG GAAGTTTGTT 901 GAAGGCCTCC CCATTAACGA CTTCTCCCGT GAGAAGATGG ACCTGACAGC AAAGGAGCTG 961 ACCGAGGAAA AGGAAACGGC TTTTGAGTTT CTCTCCTCCG CATGA
Quality Control:cDNA sequencing results is available upon request.
