Human MDFIC Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13718-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

MDFIC cDNA Clone Datasheet

MDFIC cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001166345.1
cDNA Size:	740
Gene Synonym:	HIC; MDFIC
Source:	Human

MDFIC cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens MyoD family inhibitor domain containing DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 255 C/A not causing the amino acid variation.

MDFIC cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MDFIC cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MDFIC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCGGCG CGGGCGAAGC CCTCGCTCCC GGGCCCGTGG GGCCGCAGCG CGTGGCCGAG
61       GCGGGCGGCG GCCAGCTGGG CTCCACAGCC CAGGGAAAAT GTGATAAAGA CAATACTGAG
121      AAAGATATAA CTCAAGCTAC CAATAGCCAC TTCACACATG GAGAGATGCA AGACCAGTCC
181      ATTTGGGGAA ATCCTTCGGA TGGTGAACTC ATTAGAACCC AACCTCAGCG CTTGCCTCAG
241      CTTCAGACTT CAGCACAGGT GCCAAGTGGT GAGGAAATAG GCAAGATAAA GAACGGCCAC
301      ACAGGTCTGA GCAATGGAAA TGGAATTCAC CACGGGGCCA AACACGGATC CGCAGATAAT
361      CGCAAACTTT CAGCACCTGT TTCTCAAAAA ATGCATAGAA AAATTCAGTC CAGCTTGTCT
421      GTAAACAGCG ATATCAGTAA GAAGAGCAAA GTAAATGCTG TCTTTTCCCA AAAGACAGGC
481      TCTTCACCTG AAGATTGTTG TGTCCACTGT ATCCTGGCTT GCTTGTTCTG CGAATTCCTG
541      ACCCTTTGCA ACATTGTCCT GGGACAAGCG TCATGTGGCA TCTGCACCTC AGAAGCCTGC
601      TGCTGTTGCT GTGGTGACGA GATGGGGGAT GATTGTAACT GCCCTTGTGA TATGGACTGT
661      GGCATCATGG ATGCCTGTTG TGAATCATCA GACTGCTTGG AAATCTGTAT GGAATGCTGT
721      GGAATTTGTT TTCCTTCATA

Quality Control:cDNA sequencing results is available upon request.