MD1 / MMD-1 / LY86 Protein

Lymphocyte antigen 86 Protein Datasheet

MD1 / MMD-1 / LY86 Protein Product Information

• Synonym: LY86, MD1
• Protein Construction: A DNA sequence encoding the human LY86 (O95711)(Met1-Ser162) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

MD1 / MMD-1 / LY86 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 21
• Molecular Mass: The recombinant human LY86/Fc is a disulfide-linked homodimer. The reduced monomer comprises 383 amino acids and has a predicted molecular mass of 42.7 kDa. The apparent molecular mass of the protein is approximately 48 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MD1 / MMD-1 / LY86 protein

MD1 / MMD-1 / LY86 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MD1 / MMD-1 / LY86 Protein Description

MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.

References

1. Miyake K. et al., 1995, J Immunol. 154: 3333-40.
2. Miyake K. et al., 1998, J Immunol. 61 (3): 1348-53.
3. Miura Y. et al., 1998, Blood. 92: 2815-22.
4. Nagai Y. et al., 2002, Blood. 99: 1699-705.
5. Hadidi S. et al., 2004, Tissue Antigens. 63 (2): 132-41.
6. Tsuneyoshi N. et al., 2005, J Immunol. 174 (1): 340-4.