MD-1 / LY86 Antibody

MD-1 / LY86 Antibody Datasheet

MD-1 / LY86 Antibody Product Information

• Immunogen: Recombinant Mouse MD-1 / LY86 Protein (Catalog#50062-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 416
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse MD-1 / LY86 (rM MD-1 / LY86; Catalog#50062-M08H; Met 1-Ser 162; NP_034875.1).

MD-1 / LY86 Antibody Usage Guide

• Specificity: Mouse MD-1 / LY86
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse LY86 in WB. Using a DAB detection system, the detection limit for Mouse LY86 is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse LY86. The detection limit for Mouse LY86 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

MD-1 / LY86 Antibody Background

MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.

References

1. Miyake, K. et al., 1995, J. Immunol. 154: 3333-3340.
2. Miyake, K. et al., 1998, J Immunol. 61 (3): 1348-1353.
3. Miura, Y. et al., 1998, Blood 92: 2815-2822.
4. Nagai, Y. et al., 2002, Blood 99: 1699-1705.
5. Hadidi, S. et al.,  2004, Tissue Antigens. 63 (2):132-141.
6. Tsuneyoshi, N. et al., 2005, J Immunol. 174 (1): 340-344.