Rat MBL1 Gene cDNA Clone / ORF Clone

MBL1 cDNA Clone Datasheet

MBL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012599.2
• cDNA Size: 717
• Gene Synonym: Mbpa; Mlb1;Mbl1
• Source: Rat

MBL1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus mannose-binding lectin (protein A) 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MBL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MBL1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MBL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTCCTGC TTCCACTGCT CGTCCTTCTC TGTGTAGTGA GCGTGTCCTC ATCAGGGTCA
61       CAAACCTGTG AGGAAACCCT GAAGACTTGC TCTGTGATAG CCTGCGGCAG AGACGGGAGA
121      GATGGGCCCA AAGGGGAGAA GGGAGAACCA GGTCAAGGGC TCAGGGGCTT GCAGGGCCCT
181      CCAGGGAAAC TGGGGCCTCC AGGAAGTGTA GGAGCCCCTG GAAGTCAAGG ACCAAAAGGC
241      CAAAAAGGGG ATCGTGGAGA CAGCAGAGCC ATTGAGGTGA AGCTGGCAAA TATGGAGGCA
301      GAGATAAACA CCCTGAAGTC AAAACTGGAG CTAACCAACA AGTTGCATGC CTTCTCCATG
361      GGTAAAAAGT CTGGGAAGAA GTTCTTTGTG ACCAACCATG AAAGGATGCC CTTTTCCAAA
421      GTCAAGGCCC TGTGCTCAGA GCTCCGAGGC ACTGTGGCTA TCCCCAGGAA TGCTGAGGAG
481      AACAAGGCCA TCCAAGAAGT GGCTAAAACC TCTGCCTTCC TAGGCATCAC GGACGAGGTG
541      ACTGAAGGCC AATTCATGTA TGTGACAGGG GGGAGGCTCA CCTACAGCAA CTGGAAAAAG
601      GATGAGCCCA ATGACCATGG CTCTGGGGAA GACTGTGTCA CTATAGTAGA CAACGGTCTG
661      TGGAATGACA TCTCCTGCCA AGCTTCCCAC ACGGCTGTCT GCGAGTTCCC AGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.