Human MBL Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10405-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000242.2

cDNA Size:   747

Synonym:  MBL, MBP, MBP1, COLEC1, HSMBPC, MGC116832, MGC116833, MBL2

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens mannose-binding lectin (protein C) 2, soluble (opsonic defect) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for six mutations: 378C/G not resulting in amino acid variation and the bottom TCTGA substitution by ATCTC.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGTCCCTGT TTCCATCACT CCCTCTCCTT CTCCTGAGTA TGGTGGCAGC GTCTTACTCA
61       GAAACTGTGA CCTGTGAGGA TGCCCAAAAG ACCTGCCCTG CAGTGATTGC CTGTAGCTCT
121      CCAGGCATCA ACGGCTTCCC AGGCAAAGAT GGGCGTGATG GCACCAAGGG AGAAAAGGGG
181      GAACCAGGCC AAGGGCTCAG AGGCTTACAG GGCCCCCCTG GAAAGTTGGG GCCTCCAGGA
241      AATCCAGGGC CTTCTGGGTC ACCAGGACCA AAGGGCCAAA AAGGAGACCC TGGAAAAAGT
301      CCGGATGGTG ATAGTAGCCT GGCTGCCTCA GAAAGAAAAG CTCTGCAAAC AGAAATGGCA
361      CGTATCAAAA AGTGGCTGAC CTTCTCTCTG GGCAAACAAG TTGGGAACAA GTTCTTCCTG
421      ACCAATGGTG AAATAATGAC CTTTGAAAAA GTGAAGGCCT TGTGTGTCAA GTTCCAGGCC
481      TCTGTGGCCA CCCCCAGGAA TGCTGCAGAG AATGGAGCCA TTCAGAATCT CATCAAGGAG
541      GAAGCCTTCC TGGGCATCAC TGATGAGAAG ACAGAAGGGC AGTTTGTGGA TCTGACAGGA
601      AATAGACTGA CCTACACAAA CTGGAACGAG GGTGAACCCA ACAATGCTGG TTCTGATGAA
661      GATTGTGTAT TGCTACTGAA AAATGGCCAG TGGAATGACG TCCCCTGCTC CACCTCCCAT
721      CTGGCCGTCT GTGAGTTCCC TATCTGA

Quality Control: cDNA sequencing results is available upon request.

Product Catalog

Human MBL Gene cDNA Clone / ORF Clone

MBL-2/MBL related areas, pathways, and other information