Mouse MBIP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51082-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

MBIP cDNA Clone Datasheet

MBIP cDNA Clone Product Information

Gene Bank Ref.ID:	NM_145442.2
cDNA Size:	1026
Gene Synonym:	MGC7681; 4933408E06Rik; Mbip
Source:	Mouse

MBIP cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus MAP3K12 binding inhibitory protein 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 1024-1026 TGA/ATC resulting in the amino acid Stop substitution by Ile.

MBIP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MBIP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MBIP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCTG CTGCGGAGCT TAGTTCCTCA AGCGGCAGCG AGAGGAGCCT GGAGCAATGC
61       TCCAGCCCCC TCCTCACCCG CGAGGTCCTC TGCGAAGTCT TTCGCTCCTT GCACACCCTG
121      ACAAGACAGC TTAACCTCAG AGATGATGTG GTGAAAATTA CAATCGATTG GAACAGGCTC
181      CAGAGCCTCT CAGCATCCCA GCCTGCACTG CTCCTTACTG CACTTGAACA ACACGTTTTG
241      TATTTACAGC CTTTTTTAGC AAAACTTCAG TCTTTAATGA AAGAAAACTC AACAGCTACA
301      GAGATAAGAC AGACAGAAGC AGAGACCAAG AGTGAACTAA GAGCCATACA TCCCACCGAG
361      GACCTACAAG ATGAAGGGAA GCCCAAAGAC TGTGATGTAG GAGATGTGAA AAAGACACAA
421      AACCTTTTTG ACCCAGAAGT AGTTCAGATC AAGGCTGGAA AAGCAGAAAT TGACAGACGA
481      ATATCTGCAT TTATTGAGAG AAAACAAGCT GAAATCAATG AAAACAATGT CAGGGAATTT
541      TGCAATGTTA TTGATTGTAA TCAAGAAAAT AGTTGTGCAA GAACTGATGC TGTCTTTACT
601      CCTTATCCTG GATTTAAAAG TCATGTAAAG GTTTCTAGAG TTGTGAATAC ATATGGACCA
661      CAGACTAGAC CTGAAGGAAT TGCAGGGTCA GGTCACAAAC CTACAGGCAT GCTTCGAGAT
721      TGTGGTAATC AGGCGGTAGA AGAACGACTC CAGAATATTG AGGCTCACCT GCGATTGCAG
781      ACAGGTGGCC CAGTACCAAG AGACATCTAC CAAAGAATTA AAAAGCTTGA AGATAAAATC
841      CTTGAATTGG AGGGCATCTC TCCTGAATAC TTCCAATCTG TAAACTTCTC TGGAAAAAGA
901      AGAAAAGTCC AACCACCTCA ACAGAACTAT TCACTGGCTG AACTGGATGA GAAAATTAGT
961      GCCCTCAAAC GAGCCCTGCT CAGAAAGTCA AGAGAAGCAG ACTCCATGGC CGCTCACCTT
1021     CCAATC

Quality Control:cDNA sequencing results is available upon request.