Mouse MAPKAPK3 Gene cDNA Clone / ORF Clone

MAPKAPK3 cDNA Clone Datasheet

MAPKAPK3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_178907.3
• cDNA Size: 1155
• Gene Synonym: 3PK; MK3; MAPKAP3; AI874665; MapkKapk3; Mapkapk3
• Source: Mouse

MAPKAPK3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mitogen-activated protein kinase-activated protein kinase 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations: 75 T/C and 354 T/C not causing the amino acid variation.

MAPKAPK3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MAPKAPK3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MAPKAPK3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATGGCG AGACAGCAGG GGAGAAGGGG AGTCTTGTGC CCCCGCCAGG TGCGCTCGGC
61       GGGTCCGCCT TGGGCGGTGC TCCGGCCCCA GGTGTGCGAC GGGAACCCAA GAAGTACGCG
121      GTGACTGATG ACTACCAGTT GTCCAAGCAA GTGCTGGGTC TGGGTGTGAA CGGCAAGGTA
181      CTGGAGTGCT ACCATCGGCG CTCTGGCCAG AAGTGTGCCC TGAAGCTCCT GTATGACAGC
241      CCCAAGGCCC GGCAGGAGGT GGACCACCAC TGGCAGGCGT CAGGCGGCCC CCACATCGTG
301      CGCATCCTGG ACGTGTATGA GAATATGCAC CACGGCAAGC GCTGTCTCCT CATCGTCATG
361      GAATGCATGG AGGGTGGTGA GCTGTTCAGC AGGATTCAGG AGCGTGGTGA CCAGGCTTTC
421      ACTGAGAGAG AGGCTGCAGA GATAATGCGG GACATTGGCA CTGCCATCCA GTTCTTGCAC
481      AGCCGGAACA TTGCCCACCG AGATGTCAAG CCTGAAAACC TACTCTATAC ATCCAAGGAG
541      AAGGATGCTG TACTTAAACT CACCGATTTT GGCTTTGCCA AGGAAACCAC CCAAAATGCC
601      CTCCAGACAC CCTGCTACAC TCCCTATTAT GTGGCTCCTG AGGTCCTGGG TCCAGAGAAG
661      TATGACAAGT CCTGTGATAT GTGGTCCCTG GGCGTCATCA TGTACATCCT TTTGTGTGGA
721      TTCCCACCCT TCTACTCCAA CACCGGCCAG GCCATCTCTC CAGGAATGAA AAGAAGGATT
781      CGCTTGGGCC AGTATAGCTT CCCTAACCCT GAATGGTTAG ATGTCTCTGA GGATGCCAAG
841      CAGCTAATCC GCCTGCTCCT GAAGACAGAT CCCACAGAGA GGCTAACGAT CATGCAGTTT
901      ATGAACCATC CTTGGATCAA TCAATCCATG GTGGTCCCAC AGACCCCACT CTACACAGCC
961      CGAGTGCTCC AGGAAGACAA AGATCACTGG GATGACGTCA AGGAAGAGAT GACCAGTGCC
1021     CTGGCCACTA TGCGGGTAGA CTATGACCAG GTGAAGATCA AGGACCTGAA GACCTCTAAC
1081     AACCGGCTCC TCAACAAGAG GAGGAAAAAG CAGGCAGGCA GCTCCTCAGC CTCACAAGGA
1141     TGCAACAACC AGTAA

 

Quality Control:cDNA sequencing results is available upon request.