MAPK14 / p38 alpha Protein (His Tag)

Mitogen-activated protein kinase 14 Protein Datasheet

MAPK14 / p38 alpha Protein Product Information

• Synonym: MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
• Protein Construction: A DNA sequence encoding the human MAPK14 (P63098)(Met1-Ser360) was fused with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

MAPK14 / p38 alpha Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human MAPK14 consists of 378 amino acids and has a calculated molecular mass of 43.7 kDa. The recombinant protein migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MAPK14 / p38 alpha protein

MAPK14 / p38 alpha Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MAPK14 / p38 alpha Protein Description

Mitogen-activated protein (MAP) kinases are intracellular serine/threonine kinases activated by dual phosphorylation of adjacent threonine and tyrosine, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Human p38 MAP kinase, also known as MAPK14, is activated following exposure to products of microbial pathogens, physical-chemical stimuli and cytokines. Either its autophosphorylation triggered by the interaction with MAP3K7IP1/TAB1 protein, or phosphorylation by MAP kinase kinases (MKKs) is requisite for activation of MAPK14/p38 alpha. It transduces signals by phosphorylating a number of downstream molecules including transcription factors ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, as well as tumor suppressor p53. p38 thus exists as an important regulator of both embryonic development and cancer progression. Four alternatively spliced transcript variants have been reported, and MAPK14/p38 alpha of 360 amino acids is the best characterized isoform. Furthermore, the MAPK14/p38 alpha has been suggested to play a critical role linking developmental and stress-induced erythropoiesis through regulation of Epo expression.

References

1. Han J. et al., 1995, Biochim Biophys Acta. 1265: 224-7.
2. Ben-Levy R. et al., 1998, Curr Biol. 8: 1049-57.
3. Tamura K. et al., 2000, Cell. 102: 221-31.
4. Bradham C. et al., 2006, Cell Cycle. 5: 824-8.