Mouse MAPK1 / ERK2 Protein (GST Tag)

Mitogen-activated protein kinase 1 Protein Datasheet

MAPK1 / ERK2 Protein Product Information

• Synonym: ERK; Erk2; MAPK2; PRKM2; Prkm1
• Protein Construction: A DNA sequence encoding the mouse MAPK1(P63085) (Met1-Ser358) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

MAPK1 / ERK2 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse MAPK1/GST chimera consists of 595 amino acids and has a calculated molecular mass of 69.1 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MAPK1 / ERK2 protein

MAPK1 / ERK2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MAPK1 / ERK2 Protein Description

Mitogen-activated protein kinase 1, also known as Extracellular signal-regulated kinase 2, ERK-2, and MAPK1, is a member of the protein kinase superfamily and MAP kinase subfamily. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity.

References

1. Formstecher E. et al., 2001,Dev Cell. 1 (2): 239-50.
2. Xu Be. et al., 2001, J Biol Chem. 276 (28): 26509-15.
3. Slack DN. et al., 2001, J Biol Chem. 276 (19): 16491-500.
4. Calvisi DF. et al., 2008, Cancer Res. 68 (11): 4192-200.