Anti-ERK2 Antibody

Cat: 50445-RP01
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Anti-ERK2 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ERK2 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse ERK2
Immunogen
Recombinant Mouse MAPK1 / ERK2 protein (Catalog#50445-M20B)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse MAPK1 / ERK2 (rM MAPK1 / ERK2; Catalog#50445-M20B; P63085; Met1-Ser358). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ERK2 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ERK2 Antibody Alternative Names
Anti-9030612K14Rik Antibody;Anti-AA407128 Antibody;Anti-AU018647 Antibody;Anti-C78273 Antibody;Anti-ERK Antibody;Anti-Erk2 Antibody;Anti-MAPK2 Antibody;Anti-p41mapk Antibody;Anti-p42mapk Antibody;Anti-Prkm1 Antibody;Anti-PRKM2 Antibody
ERK2 Background Information

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 are implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as Mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
mitogen-activated protein kinase 1
Research Areas
References
  • Houslay MD, et al. (2003) The role of ERK2 docking and phosphorylation of PDE4 cAMP phosphodiesterase isoforms in mediating cross-talk between the cAMP and ERK signalling pathways. Biochem Soc Trans. 31(Pt 6): 1186-90.
  • Jivan A, et al. (2010) Reconstitution of the Nuclear Transport of the MAP Kinase ERK2. Methods Mol Biol. 661: 273-85.
  • Yu CG, et al. (2010) Involvement of ERK2 in traumatic spinal cord injury. J Neurochem. 113(1): 131-42.
  • Subramaniam S, et al. (2010) ERK and cell death: ERK1/2 in neuronal death. FEBS J. 277(1): 22-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ERK2 Antibody Rabbit Polyclonal WB,ELISA 50445-T48
    Anti-ERK2 Antibody Rabbit Monoclonal ELISA,FCM,ICC/IF,IF 10030-R007
    Anti-ERK2 Antibody Mouse Monoclonal WB,ELISA 10030-MM09
    Anti-ERK2 Antibody Rabbit Polyclonal ELISA 10030-RP01
    Anti-ERK2 Antibody Rabbit Polyclonal WB,ELISA,IP 10030-T52
    Anti-ERK2 Antibody Rabbit Monoclonal ELISA 10030-R006
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