Mouse MAP3K3/MEKK3 Gene cDNA Clone / ORF Clone

MAP3K3/MEKK3 cDNA Clone Datasheet

MAP3K3/MEKK3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011947.3
• cDNA Size: 1881
• Gene Synonym: Mekk3; MAPKKK3; AW548911; KIAA4031; mKIAA4031; Map3k3
• Source: Mouse

MAP3K3/MEKK3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus mitogen-activated protein kinase kinase kinase 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

MAP3K3/MEKK3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

MAP3K3/MEKK3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

MAP3K3/MEKK3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATGAAC AAGAGGCATT AGACTCGATC ATGAAGGACC TGGTGGCCCT CCAGATGAGC
61       CGACGAACCC GGTTGTCTGG ATATGAGACC ATGAAGAATA AGGACACAGG TCACCCAAAC
121      AGGCAGAGTG ACGTCAGAAT CAAGTTTGAA CACAATGGGG AGAGACGAAT TATAGCATTC
181      AGCCGGCCTG TGAGATACGA AGATGTGGAG CACAAGGTGA CAACAGTCTT TGGGCAGCCT
241      CTTGATTTGC ATTATATGAA TAATGAGCTC TCCATCCTGT TGAAAAACCA AGATGATCTC
301      GATAAAGCCA TTGACATTTT GGATAGAAGC TCAAGTATGA AAAGCCTTAG GATACTACTG
361      TTATCCCAAG ACAGAAACCA TACTAGTTCC TCTCCCCACT CTGGAGTGTC CAGGCAGGTT
421      CGGATCAAGC CTTCCCAGTC TGCAGGGGAT ATAAATACCA TCTACCAAGC TCCTGAGCCC
481      AGAAGCAGGC ACCTGTCTGT CAGCTCCCAG AACCCTGGCC GAAGCTCTCC TCCCCCGGGA
541      TATGTACCTG AGCGACAACA GCACATTGCC CGGCAAGGAT CCTATACGAG CATCAACAGC
601      GAAGGTGAAT TCATCCCAGA GACCAGCGAA CAGTGTATGC TAGATCCCCT CAGCAGTGCC
661      GAAAATTCCT TGTCAGGAAG CTGCCAATCC TTGGACAGGT CAGCAGACAG CCCATCCTTC
721      AGGAAATCAC AAATGTCCCG AGCCCGGAGC TTCCCAGACA ACAGAAAGGA ATGCTCAGAT
781      CGGGAGACCC AGCTCTATGA TAAAGGTGTC AAAGGTGGAA CCTATCCCAG GCGCTACCAT
841      GTGTCTGTGC ATCACAAAGA CTACAATGAT GGCAGAAGAA CATTTCCCCG AATACGACGG
901      CATCAAGGCA ACCTATTCAC TCTGGTGCCC TCAAGTCGCT CCTTGAGCAC AAATGGCGAG
961      AACATGGGTG TAGCTGTGCA ATACCTGGAC CCCCGTGGGC GCCTACGGAG TGCAGACAGT
1021     GAGAATGCCC TCACTGTGCA GGAAAGGAAT GTGCCAACCA AATCTCCTAG TGCTCCCATC
1081     AATTGGCGTC GGGGGAAGCT CCTGGGTCAA GGTGCCTTCG GCAGGGTCTA CTTGTGCTAT
1141     GATGTGGACA CAGGACGTGA ACTTGCTTCT AAGCAGGTCC AGTTTGACCC AGATAGTCCT
1201     GAGACAAGCA AGGAGGTGAG TGCTCTGGAG TGTGAGATCC AGTTGCTGAA GAACCTGCAG
1261     CATGAGCGCA TTGTGCAGTA CTACGGCTGC CTGCGGGACC GTGCTGAGAA GATCCTCACC
1321     ATCTTTATGG AGTATATGCC AGGGGGCTCT GTAAAAGACC AGTTGAAGGC CTACGGAGCT
1381     CTGACAGAGA GTGTGACCCG CAAGTACACC CGGCAGATTC TGGAGGGCAT GTCATACCTG
1441     CACAGCAACA TGATTGTGCA TCGGGACATC AAGGGAGCCA ATATCCTCCG AGACTCAGCT
1501     GGGAATGTGA AGCTTGGGGA TTTTGGGGCC AGCAAACGCC TACAGACCAT CTGCATGTCA
1561     GGGACAGGCA TTCGCTCTGT CACTGGCACA CCCTACTGGA TGAGTCCTGA AGTCATCAGT
1621     GGCGAGGGCT ATGGAAGAAA GGCAGACGTG TGGAGCCTGG GCTGTACTGT GGTGGAAATG
1681     CTGACAGAGA AACCACCTTG GGCAGAGTAT GAAGCTATGG CTGCCATTTT CAAGATTGCC
1741     ACCCAGCCTA CCAATCCTCA GCTGCCCTCT CACATCTCAG AACACGGCAG GGACTTCCTG
1801     AGGCGCATAT TTGTGGAAGC TCGTCAGAGA CCCTCAGCTG AGGAGCTGCT CACACACCAC
1861     TTTGCACAGC TAGTGTACTG A

 

Quality Control:cDNA sequencing results is available upon request.