|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Mus musculus mitogen-activated protein kinase kinase kinase 13 DNA.|
|LZK, C130026N12Rik, Map3k13|
|HindIII + XhoI|
|Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG|
|Identical with the Gene Bank Ref. ID sequence except for two point mutations 1637 A/G resulting in the amino acid Lys substitution by Arg;1655 G/A resulting in the amino acid Arg substitution by Gln.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone)||MG50831-G|
|Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||MG50831-G-F|
|Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||MG50831-G-H|
|Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||MG50831-G-N|
|Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||MG50831-G-Y|
|Product name||Product name|