Text Size:AAA

Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
MAP3K13cDNA Clone Product Information
Gene Bank Ref.ID:NM_172821.3
cDNA Size:2910
cDNA Description:ORF Clone of Mus musculus mitogen-activated protein kinase kinase kinase 13 DNA.
Gene Synonym:LZK, C130026N12Rik, Map3k13
Species:Mouse
Vector:pCMV/hygro-Myc
Restriction Site:HindIII + XhoI
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations 1637 A/G resulting in the amino acid Lys substitution by Arg;1655 G/A resulting in the amino acid Arg substitution by Gln.
Shipping Carrier:Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.
Storage:The Whatman FTA elute card can be stored at room temperature for three months under dry condition.
Image
Mouse MAP3K13 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged

pCMV/hygro-Myc vector information
Vector Name pCMV/hygro-Myc
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag Myc
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV/hygro-Myc Physical Map

Schematic of pCMV/hygro-Myc Multiple Cloning Sites

Myc tag info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Related Products
Product nameProduct name
Background
References
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"