MKK6 Protein, Human, Recombinant

Cat: 10422-HNCB

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MKK6 Protein, Human, Recombinant Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Kinase activity untested
Protein Construction
A DNA sequence encoding the human MKK6 (NP_002749.2) (Met 1-Asp334) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Gly
Molecule Mass
The secreted recombinant human MKK6 consists of 336 amino acids and predicts a molecular mass of 37.6 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MKK6 Protein, Human, Recombinant images

Human MKK6/MEK6/MAP2K6 Protein 8798

MKK6 Protein, Human, Recombinant Alternative Names

MAPKK6 Protein, Human;MEK6 Protein, Human;MKK6 Protein, Human;PRKMK6 Protein, Human;SAPKK-3 Protein, Human;SAPKK3 Protein, Human

MKK6 Background Information

Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Full Name
mitogen-activated protein kinase kinase 6
References
  • Raingeaud J., et al.,(1996), MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Mol. Cell. Biol. 16:1247-1255.
  • Stein B., et al., (1996), Cloning and characterization of MEK6, a novel member of the mitogen-activated protein kinase kinase cascade.J. Biol. Chem. 271:11427-11433.
  • Han J., et al.,(1996), Characterization of the structure and function of a novel MAP kinase kinase (MKK6).J. Biol. Chem. 271:2886-2891.
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