MAP1D Antibody

MAP1D Antibody Datasheet

MAP1D Antibody Product Information

• Immunogen: Recombinant Human MAP1D protein (Catalog#10883-H08B)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 008
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human MAP1D (rh MAP1D; Catalog#10883-H08B; NP_954697.1; Arg 44-Ala 335).

MAP1D Antibody Usage Guide

• Specificity: Human MAP1D
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human MAP1D. The detection limit for Human MAP1D is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

MAP1D Antibody Background

Methionine aminopeptidase 1D, also known as MAP1D, is a member of thepeptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesi.

References

1. Serero, A. et al., 2003, J Biol Chem. 278 (52): 52953-63.
2. Leszczyniecka, M. et al., 2006, Oncogene. 25 (24): 3471-8.
3. Selvakumar, P. et al., 2006,Biochim Biophys Acta. 1765 (2): 148-54.
4. Hu, XV. et al., 2007, Biochemistry. 46: 12833-12843.