Anti-METAP1D Antibody

Cat: 10883-R008
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Anti-METAP1D Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-METAP1D Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human METAP1D
Immunogen
Recombinant Human MAP1D protein (Catalog#10883-H08B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human MAP1D (rh MAP1D; Catalog#10883-H08B; NP_954697.1; Arg 44-Ala 335).
Source
Monoclonal Rabbit IgG Clone #008
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-METAP1D Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-METAP1D Antibody Alternative Names
Anti-MAP 1D Antibody;Anti-MAP1D Antibody;Anti-MetAP 1D Antibody;Anti-Metap1l Antibody
METAP1D Background Information

Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.

Full Name
methionyl aminopeptidase type 1D (mitochondrial)
References
  • Serero A., et al.,(2003), An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway. J. Biol. Chem. 278:52953-52963.
  • Leszczyniecka M., et al., (2006), MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer.Oncogene 25:3471-3478.
  • Vaca Jacome A.S., et al.,(2015), N-terminome analysis of the human mitochondrial proteome.Proteomics 15:2519-2524.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-METAP1D Antibody Mouse Monoclonal ELISA 10883-MM04
    Anti-METAP1D Antibody Rabbit Polyclonal ELISA 10883-RP02
    Anti-METAP1D Antibody Rabbit Polyclonal ELISA 10883-T16
    Anti-METAP1D Antibody Rabbit Polyclonal ELISA 10883-RP01
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