MAOA / Monoamine oxidase A (aa1-497) Protein (GST Tag)

Monoamine oxidase A Protein Datasheet

MAOA / Monoamine oxidase A Protein Product Information

• Synonym: RP1-201D17__B.2
• Protein Construction: A DNA sequence encoding the human MAOA (P21397) cytoplasmic domain (Met 1-Ser 497) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

MAOA / Monoamine oxidase A Protein QC Testing

• Purity: >84% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human MAOA (aa 1-497)/GST chimera consists of 735 amino acids and has a calculated molecular mass of 84.2 KDa. It migrates as an approximately 75KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH 8.0, 10%glycerol
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: MAOA / Monoamine oxidase A protein

MAOA / Monoamine oxidase A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MAOA / Monoamine oxidase A Protein Description

Monoamine oxidase A, also known as MAOA, is an enzyme belonging to the monoamine oxidase family. MAOA preferentially degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin and is inhibited by clorgiline and befloxatone. This enzyme localizes to the mitochondrial outer membrane. MAOA is found bound to the outer membrane of mitochondria in most cell types in the body. Both of the two subtypes of monoamine oxidase, MAOA and MAOB are found in neurons and astroglia, with MAOA also found in the liver, gastrointestinal tract and placenta. Abnormal regulation of MAOA in the body has been associated with depression, substance abuse, attention deficit disorder, and irregular sexual maturation. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.

References

1. Huang SY. et al., 2009, World J Biol Psychiatry. 10 (4): 544-51.
2. Guo G. et al., 2008, Eur J Hum Genet. 16 (5): 626-34.
3. Mc Dermott R. et al., 2009, Proc Natl Acad Sci. 106 (7): 2118-23.